CAH6 | Carbonic anhydrase 6

Name: CAH6 | Carbonic anhydrase 6
SKU: AS19 4322
Price: 302 EUR
Availability: InStock

AS19 4322 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamdydomonas reinhardtii

CAH6 | Carbonic anhydrase 6 in the group Antibodies Plant/Algal / Photosynthesis / RUBISCO/Carbon metabolism at Agrisera AB (Antibodies for research) (AS19 4322)

Product Information

Immunogen Recombinant, full length CAH6 of Chlamydomonas reinhardtii, excised from the gel, UniProt: Q6S7R9

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 28 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Gonium pectorale, Volvox carteri
Species of your interest not listed? Contact us

Not reactive in Neopyropia yezoensis (macroalga)

Application examples

Additional information

Lately localization to flagella has been confirmed for CAH6 Mackinder et al. 2017.

Extraction method – harvest the cells and add SDS sample buffer.

Background

Background CAH6 (Carbonic anhydrase 6) is a zinc-containing metalloenzymes that catalyze the reversible hydration of CO₂. There are three evolutionary not related families of carbonic anhydrases: alpha, beta and gamma. CAH6 belonds to the beta family and initially was localized to chloroplasts Mitra et al. (2004).
However, lately localization to flagella has been confirmed Mackinder et al. 2017.

Product citations

Selected references Mackinder et al. 2017. A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Cell. 2017 Sep 21;171(1):133-147.e14. doi: 10.1016/j.cell.2017.08.044.
Mitra et al. (2004). Identification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 2004 May;135(1):173-82.

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immunogen:	Recombinant, full length CAH6 of Chlamydomonas reinhardtii, excised from the gel, UniProt: Q6S7R9
Reconstitution:	For reconstitution add 50 µl, of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	28 kDa
Confirmed reactivity:	Chlamydomonas reinhardtii
predicted reactivity:	Gonium pectorale, Volvox carteri Species of your interest not listed? Contact us
not reactive in:	Neopyropia yezoensis (macroalga)
additional information (application):	Lately localization to flagella has been confirmed for CAH6 Mackinder et al. 2017. Extraction method – harvest the cells and add SDS sample buffer.
background:	CAH6 (Carbonic anhydrase 6) is a zinc-containing metalloenzymes that catalyze the reversible hydration of CO₂. There are three evolutionary not related families of carbonic anhydrases: alpha, beta and gamma. CAH6 belonds to the beta family and initially was localized to chloroplasts Mitra et al. (2004). However, lately localization to flagella has been confirmed Mackinder et al. 2017.
All references:	Mackinder et al. 2017. A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Cell. 2017 Sep 21;171(1):133-147.e14. doi: 10.1016/j.cell.2017.08.044. Mitra et al. (2004). Identification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 2004 May;135(1):173-82.