CAH6 | Carbonic anhydrase 6
AS19 4322 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamdydomonas reinhardtii

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Recombinant, full length CAH6 of Chlamydomonas reinhardtii, excised from the gel, UniProt: Q6S7R9
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 ĩl
Reconstitution
For reconstitution add 50 ĩl, of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
28 kDa
Reactivity
Confirmed reactivity
Chlamydomonas reinhardtii
Predicted reactivity
Gonium pectorale, Volvox carteri
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Neopyropia yezoensis (macroalga)
Application examples
Additional information
Lately localization to flagella has been confirmed for CAH6 Mackinder et al. 2017.
Extraction method – harvest the cells and add SDS sample buffer.
Extraction method – harvest the cells and add SDS sample buffer.
Background
Background
CAH6 (Carbonic anhydrase 6) is a zinc-containing metalloenzymes that catalyze the reversible hydration of CO2. There are three evolutionary not related families of carbonic anhydrases: alpha, beta and gamma. CAH6 belonds to the beta family and initially was localized to chloroplasts Mitra et al. (2004).
However, lately localization to flagella has been confirmed Mackinder et al. 2017.
However, lately localization to flagella has been confirmed Mackinder et al. 2017.
Product citations
Selected references
Mackinder et al. 2017. A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Cell. 2017 Sep 21;171(1):133-147.e14. doi: 10.1016/j.cell.2017.08.044.
Mitra et al. (2004). Identification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 2004 May;135(1):173-82.
Mitra et al. (2004). Identification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 2004 May;135(1):173-82.
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