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CAH6 | Carbonic anhydrase 6

AS19 4322 | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Chlamdydomonas reinhardtii

CAH6 | Carbonic anhydrase 6 in the group Antibodies for Plant/Algal  / Photosynthesis  / RUBISCO/Carbon metabolism at Agrisera AB (Antibodies for research) (AS19 4322)

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Data sheet Product citations Protocols Add review

Product Information

Immunogen Recombinant, full length CAH6 of Chlamydomonas reinhardtii, excised from the gel, UniProt: Q6S7R9
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 28 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Gonium pectorale, Volvox carteri
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Lately localization to flagella has been confirmed for CAH6 Mackinder et al. 2017.

Extraction method – harvest the cells and add SDS sample buffer.

Related products

Related products AS05 073 | Anti-CAH3 | Carbonic anhydrase, rabbit antibodies
AS11 1737 | Anti-Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas, rabbit antibodies
AS17 4114 | Anti-Gamma CA | Gamma Carbonic anhydrases

Background

Background CAH6 (Carbonic anhydrase 6) is a zinc-containing metalloenzymes that catalyze the reversible hydration of CO2. There are three evolutionary not related families of carbonic anhydrases: alpha, beta and gamma. CAH6 belonds to the beta family and initially was localized to chloroplasts Mitra et al. (2004).
However, lately localization to flagella has been confirmed Mackinder et al. 2017.

Product citations

Selected references Mackinder et al. 2017. A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Cell. 2017 Sep 21;171(1):133-147.e14. doi: 10.1016/j.cell.2017.08.044.
Mitra et al. (2004).  Identification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 2004 May;135(1):173-82.

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