Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas

Name: Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas
SKU: AS11 1737
Price: 394 EUR
Availability: InStock

AS11 1737 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii | marker of mitochondria for low carbon dioxide grown cells

Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas in the group Antibodies Plant/Algal / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS11 1737)

Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas

Product Information

Immunogen

recombinant Chlamydomonas reinhardtii mitochondrial CA, as described in Villand et al. 1997. Accession number Q39590 and Q39589

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunofluorescence (IF), Western blot (WB)

Recommended dilution 1 : 200 (IF), 1 : 1000 (WB)

Expected | apparent MW 23,7 | 21-22 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Chlamydomonas reinhardtii

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody is recognizing both isoforms, beta- CA1 and beta-CA2 and can be used as mitochondrial marker for low carbon dioxide grown cells of Chlamydomonas reinhardtii

Background

Beta-CA1, beta-CA2 is a low-CO2-induced mitochondrial carbonic anhydrase. that catalyze the reversible interconversion of carbon dioxide and water to bicarbonate and protons. Localised into mitochondrial stroma.

Alternative name: MtCA.

Product citations

Selected references Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9
Kuken et al. (2018). Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts. Elife. 2018 Oct 11;7. pii: e37960. doi: 10.7554/eLife.37960.
Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015.
Tirumani et al. (2014). Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light-dark cycles in synchronous cultures. Plant Mol Biol. 2014 Mar 4.
Renberg et al. (2010). A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii. Plant physiol. 154: 187-196.

immunogen:	recombinant Chlamydomonas reinhardtii mitochondrial CA, as described in Villand et al. 1997. Accession number Q39590 and Q39589
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	200 µl
Reconstitution:	For reconstitution add 200 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunofluorescence (IF), Western blot (WB)
recommended dilution:	1 : 200 (IF), 1 : 1000 (WB)
Expected \| apparent MW:	23,7 \| 21-22 kDa
Confirmed reactivity:	Chlamydomonas reinhardtii
predicted reactivity:	Chlamydomonas reinhardtii
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Antibody is recognizing both isoforms, beta- CA1 and beta-CA2 and can be used as mitochondrial marker for low carbon dioxide grown cells of Chlamydomonas reinhardtii
background:	Beta-CA1, beta-CA2 is a low-CO2-induced mitochondrial carbonic anhydrase. that catalyze the reversible interconversion of carbon dioxide and water to bicarbonate and protons. Localised into mitochondrial stroma. Alternative name: MtCA.
All references:	Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9 Kuken et al. (2018). Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts. Elife. 2018 Oct 11;7. pii: e37960. doi: 10.7554/eLife.37960. Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015. Tirumani et al. (2014). Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light-dark cycles in synchronous cultures. Plant Mol Biol. 2014 Mar 4. Renberg et al. (2010). A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii. Plant physiol. 154: 187-196.

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