AOX1 | Algal Alternative oxidase 1

Product no: AS06 152

AS06 152  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii  |  cellular [compartment marker] of mitochondrial inner membrane

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  • Product Info
  • Immunogen:

    whole presumed mature AOX1 protein from from Chlamydomonas reinhardtii UniProt: O65000 fused to GST

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 10 000 (WB)
    Expected | apparent MW:

    36 | 36 kDa (Chlamydomonas reainhardii)

  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii
    Predicted reactivity: Aspergilus niger, Gonium pectorale, Monoraphidium neglectum, Nannochloropsis gaditana, Ostreococcus lucimarinus, Tetrabaena socialis, Volvox carteri f. nagariensis

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Cellular [compartment marker] of Chlamydomonas reinhardtii mitochondrial inner membrane
  • Background
  • Background:

    The mitochondrial AOX (alternative oxidase) of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 and Aox2. The  alternative respiratory pathway is comprised of a single homodimeric protein – AOX – and functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport.  Alternative oxidase expression is  influenced by different stress stimuli.

  • Product Citations
  • Selected references: Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9
    Gu et al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtii
    Perlaza et al. (2019). The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577.
    Kaye et al. (2019). The mitochondrial alternative oxidase from Chlamydomonas reinhardtii enables survival in high light.J Biol Chem. 2019 Jan 25;294(4):1380-1395. doi: 10.1074/jbc.RA118.004667.
    Zalutskaya et al. (2015). The Chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress. Plant Physiol Biochem. 2015 Dec;97:229-34. doi: 10.1016/j.plaphy.2015.10.014.
    Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
    Inman (2013). Characterization of the Alternative Oxidase from the Psychrophilic Green Alga Chlamydomonas sp. UWO241. Thesis.
    Bohne et al. (2013). Reciprocal Regulation of Protein Synthesis and Carbon Metabolism for Thylakoid Membrane Biogenesis. PLOS, Open Access.
  • Protocols
  • Isolation of mitochondrial fraction

    Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
  • Reviews:
  • To leave a review, you should have previously purchased this product and to your customer profile.

    Marie Bertrand | 2023-09-20
    0 | 0
    Used on chlamydomonas. Good signal.
    Andrew Castonguay | 2020-08-08
    0 | 0
    Loaded 20 ug of C. reinhardtii whole cell extract from freeze thaw method. Blocked with TBS+0.1%Tween, 5% milk for 1 hr at rm temp or overnight at 4 deg C. Antibody diluted 1:10,000 in TBS+0.1%Tween, 2% milk. Primary incubation overnight with gentle agitation at 4 deg C. 5 washes in between primary and secondary, volume for all antibody incubations and washes was 25 ml, washes at room temp. Immunoblot with HRP-conjugated secondary (1:20,000, 0.1%TBST) was clean with these conditions, however, longer exposure will yield a few additional bands, one at ~60 kDa and another at ~30 kDa (12% polyacrylamide).

Accessories

AS06 151  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii  |  cellular [compartment marker] of mitochondrial inner membrane

302 €
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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
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AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

206 €
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AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S

This product can be purchased in 3 different volumes:

AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer

AS16 ECL-SN, 100 ml

AS16 ECL-SN-1L, 1L

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From 26 €
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