AOX1 | Algal Alternative oxidase 1

AS06 152 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of mitochondrial inner membrane

Product Information

Immunogen

whole presumed mature AOX1 protein from from Chlamydomonas reinhardtii UniProt: O65000 fused to GST

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 10 000 (WB)

Expected | apparent MW

36 | 36 kDa (Chlamydomonas reainhardii)

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Aspergilus niger, Gonium pectorale, Monoraphidium neglectum, Ostreococcus lucimarinus, Tetrabaena socialis, Volvox carteri f. nagariensis

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Cellular [compartment marker] of Chlamydomonas reinhardtii mitochondrial inner membrane

Related products

AS04 054 | Anti-plant alternative oxidase 1 and 2, polyclonal rabbit antibodies

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Background

The mitochondrial AOX (alternative oxidase) of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 and Aox2. The alternative respiratory pathway is comprised of a single homodimeric protein – AOX – and functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport. Alternative oxidase expression is influenced by different stress stimuli.

Product citations

Selected references Perlaza et al. (2019). The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577.
Kaye et al. (2019). The mitochondrial alternative oxidase from Chlamydomonas reinhardtii enables survival in high light.J Biol Chem. 2019 Jan 25;294(4):1380-1395. doi: 10.1074/jbc.RA118.004667.
Zalutskaya et al. (2015). The Chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress. Plant Physiol Biochem. 2015 Dec;97:229-34. doi: 10.1016/j.plaphy.2015.10.014.
Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
Inman (2013). Characterization of the Alternative Oxidase from the Psychrophilic Green Alga Chlamydomonas sp. UWO241. Thesis.
Bohne et al. (2013). Reciprocal Regulation of Protein Synthesis and Carbon Metabolism for Thylakoid Membrane Biogenesis. PLOS, Open Access.