COXIIb | Algal Cytochrome oxidase subunit II b
AS06 151 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of mitochondrial inner membrane
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GST fusion with the aminoacids 4-153 of the subunit 2b of the Chlamydomonas reinhardtii cytochrome oxidase, UniProt: Q9AU05
Chlamydomonas reinhardtii membrane extract (A), Chlamydomonas reinhardtii total cell extract, prepared by sonication, loading 14 µl equivalent to 30 µg of total protein (B), Chlamydomonas reinhardtii total cell extract, rapid, prepared directly by spinning down the cells and lysis of cell pellet in SDS-PAGE sample buffer and loading 14 µl equivalent to 98 µg of total protein (C), denatured at 100°C for 5 min. were separated on 15 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 1 % blocking buffer (2 ml blocking reagent stock solution ROCHE 11 520 709 001 in 20 ml TBS) for ON at 4°C without agitation. Blot was incubated in the primary antibody at a dilution of 1: 25 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed using chemiluminescent detection reagent according to manufacture instructions. Exposure time was 8 seconds.
Courtesy of Nadine Coosemans, Laboratoire de génétique et physiologie des microalgues, Université de Liège, Belgium
COXII - mitochondrial cytochrome oxidase subunit II is located in inner mitochondrial membrane. The enzyme is a component of the respiratory chain complex involved in the transfer of electrons from cytochrome c to oxygen.
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