COXII | Plant Cytochrome oxidase subunit II (affinity purified)

AS04 053A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Eudicots, monocots, Physcomitrella patens | Cellular [compartment marker] of mitochondrial inner membrane

Product Information

Immunogen

KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum

Format Lyophilized in PBS pH 7.4

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Blue Native-PAGE (BN-PAGE), Western blot (WB)

Recommended dilution 1 : 1000 (BN-PAGE), 1 : 1000 (WB)

Expected | apparent MW

29.4 | 30 kDa (for Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Cucumis sativus, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrella patens, Triticum aestivum, Triticum durum Desf. , Zea mays, Vicia faba, Quercus rubra

Predicted reactivity Cucumis melo, Glycine max, Oryza sativa, Physcomitrella patens, Pisum sativum, Triticum aestivum, Vigna radiata
Species of your interest not listed? Contact us

Not reactive in Saccharina japonica

Application examples

Additional information

Cellular [compartment marker] of mitochondrial inner membrane

Antibody detects COXII protein most optimally in membrane fractions. The signal is weak in a in total protein extract.

Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

Related products

Plant protein extraction buffer

Background

Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

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