Name:
Phone:
E-mail:
Address:


 


COXII | Plant Cytochrome oxidase subunit II (affinity purified)

AS04 053A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Eudicots, monocots, Physcomitrella patens  |  Cellular [compartment marker] of mitochondrial inner membrane

COXII | Plant Cytochrome oxidase subunit II (affinity purified) in the group Antibodies for Plant/Algal  / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS04 053A)

DATA SHEET IN PDF

Qty: 
292
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including  Oryza sativa P04373 and Physcomitrella patens Q1XGA9

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Blue Native-PAGE (BN-PAGE), Western blot (WB)
Recommended dilution 1 : 1000 (BN-PAGE), 1 : 1000 (WB)
Expected | apparent MW

29.4 | 30 kDa (for Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Cucumis sativus, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrella patens, Triticum aestivum, Triticum durum Desf. , Zea mays, Vicia faba, Quercus rubra
Predicted reactivity Cucumis melo, Glycine max, Oryza sativa, Physcomitrella patens, Pisum sativum, Triticum aestivum, Vigna radiata
Species of your interest not listed? Contact us
Not reactive in Saccharina japonica

Application examples

Additional information

Additional information

Cellular [compartment marker] of mitochondrial inner membrane

Antibody detects COXII protein most optimally in membrane fractions. The signal is weak in a in total protein extract.

Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

Related products

Related products
AS04 052 | Anti-COXII (plant),  chicken antibodies
AS04 053A-200 | Anti-COXII | cytochrome oxidase subunit II (plant), rabbit antibodies
AS04 053PRE | COXII | cytochrome oxidase subunit II, pre-immune serum
AS04 053P COXII | cytochrome oxidase subunit II | Blocking peptide

Antibodies to other mitochondrial proteins

Background

Background

Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

Product citations

Related products: COXII | Plant Cytochrome oxidase subunit II (affinity purified)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS04 053A-200  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactiv...
741 €
AS04 052  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: A. ...
381 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Off...
194 €