CBP80 | nuclear cap-binding protein subunit 1
AS09 531 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, B.oleracea, P.endiviifolia
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Product Information
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
200 µl
Reconstitution
For reconstitution add 200 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
RNA Immunoprecipitation (RIP), Western blot (WB)
Recommended dilution
5-10 µg (RIP), 1 : 1000 (WB)
Expected | apparent MW
96.5 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana,Brassica oleracea, Pellia endiviifolia
Predicted reactivity
Solanum tuberosum, Ricinus communis
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Suggested blotting conditions: 8% gel, semi dry-blotting system, 25 V, 75 min, PVDF membrane.
Background
Background
CBP80 is a component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing. Alternative names: 80 kDa nuclear cap-binding protein, NCBP 80 kDa subunit, abscisic acid-hypersensitive protein 1, ABA-hypersensitive protein 1, Protein ENSALADA
Product citations
Selected references
Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018. (RNA immunoprecipiation)
Raczynska et al. (2013). TheSERRATEprotein isinvolved inalternativesplicing inArabidopsis thaliana. Nucleic Acids Res. Oct 16.
Raczynska et al. (2013). TheSERRATEprotein isinvolved inalternativesplicing inArabidopsis thaliana. Nucleic Acids Res. Oct 16.
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