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CBP20 | nuclear cap-binding protein subunit 2

AS09 530 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

CBP20 | nuclear cap-binding protein subunit 2 in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / plant RNA at Agrisera AB (Antibodies for research) (AS09 530)
CBP20 | nuclear cap-binding protein subunit 2



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Product Information

Immunogen

KLH-conjugated peptide, derived with Arabidopsis thaliana CBP20 protein Q9xFD1, At5g44200

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 29,6 | 30 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Glycne max, Hordeum vulgare, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Zea mays
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example

western blot detection of plant CBP20


25 µg of total protein extratcs from 10 days old seedlings of Arabidopsis thaliana  (wild type and a CBP20 mutant) were separated on 12.5 % SDS-PAGE and blotted 1h to PVDF (tank blotting). Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 30 min.


Courtesy of Dr. Sascha Laubinger, ZMBP, Germany

Reactant: Saccharomyces cerevisiae (Yeast)

Application: Western Blotting

Pudmed ID: 29755485

Journal: Front Plant Sci

Figure Number: 6B

Published Date: 2018-05-15

First Author: Pieczynski, M., Kruszka, K., et al.

Impact Factor: 5.435

Open Publication

The localization, lack or replacement of the U12 intron in the CBP20 gene does not influence the total level of the CBP20 transcript but impacts the level of CBP20 protein in plants. (A) Real-time qPCR analysis of the total level of the CBP20 transcript in maxi-gene transgenic lines. For each construct, two independent transgenic lines were analyzed. Wt – wild-type plants; cbp20 – cbp20 mutant line; wt transgene – wild-type CBP20 gene structure; U12?U2 – CBP20 gene in which the original U12 intron was replaced with the U2 intron derived from the Arabidopsis CBP80 gene; ?U12 – CBP20 gene with U12 intron deletion; exon swap – the CBP20 gene in which exons no. 4 and no. 5 flanking the U12 intron have been exchanged; U12 in core - a derivative of the U12?U2 construct in which the U12 intron has been introduced between exons no. 3 and no. 4; and U12 in tail - derivative of the U12?U2 construct in which the U12 intron has been introduced between exons no. 5 and no. 6. The CBP20 mRNA level in Wt was taken as 1. Values are shown as the mean ą SD (n = 3) from three independent experiments. (B) Western blot analysis of CBP20 protein levels in transgenic Arabidopsis plants. For each construct, two independent transgenic lines were analyzed. Upper panel – immunoblot using antibodies against CBP20 protein; lower panel – immunoblot with antibodies against actin used as a loading control. Numbers below the western blot image are relative intensities of CBP20 bands calculated using the wt transgene (line 1) CBP20 level as 1. Lines are described as previously.

Additional information

Related products

Related products

AS09 531 | Anti/CBP80 | nuclear cap-binding protein subunit 1, rabbit antibodies

microRNA antibodies

antibodies to microRNA targets

Plant protein extraction buffer

Secondary antibodies

Background

Background

CBP20 (Nuclear cap-binding protein subunit 2)is a component of the cap-binding complex (CBC), involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). Alternative names: 20 kDa nuclear cap-binding protein,  NCBP 20 kDa subunit

Product citations

Selected references Raczynska et al. (2013). TheSERRATEprotein isinvolved inalternativesplicing inArabidopsis thaliana. Nucleic Acids Res. Oct 16.

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