Anti-CBP20 | nuclear cap-binding protein subunit 2

Product no: AS09 530

AS09 530 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated peptide, derived with Arabidopsis thaliana CBP20 protein Q9xFD1, At5g44200

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 200 µg
    Reconstitution: For reconstitution add 200 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 29.6 | 30 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Glycne max, Hordeum vulgare, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Zea mays

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot detection of plant CBP20

    25 µg of total protein extratcs from 10 days old seedlings of Arabidopsis thaliana  (wild type and a CBP20 mutant) were separated on 12.5 % SDS-PAGE and blotted 1h to PVDF (tank blotting). Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 30 min.

    Courtesy of Dr. Sascha Laubinger, ZMBP, Germany

    Application examples:

    Reactant: Saccharomyces cerevisiae (Yeast)

    Application: Western Blotting

    Pudmed ID: 29755485

    Journal: Front Plant Sci

    Figure Number: 6B

    Published Date: 2018-05-15

    First Author: Pieczynski, M., Kruszka, K., et al.

    Impact Factor: 5.435

    Open Publication

    The localization, lack or replacement of the U12 intron in the CBP20 gene does not influence the total level of the CBP20 transcript but impacts the level of CBP20 protein in plants. (A) Real-time qPCR analysis of the total level of the CBP20 transcript in maxi-gene transgenic lines. For each construct, two independent transgenic lines were analyzed. Wt – wild-type plants; cbp20 – cbp20 mutant line; wt transgene – wild-type CBP20 gene structure; U12?U2 – CBP20 gene in which the original U12 intron was replaced with the U2 intron derived from the Arabidopsis CBP80 gene; ?U12 – CBP20 gene with U12 intron deletion; exon swap – the CBP20 gene in which exons no. 4 and no. 5 flanking the U12 intron have been exchanged; U12 in core - a derivative of the U12?U2 construct in which the U12 intron has been introduced between exons no. 3 and no. 4; and U12 in tail - derivative of the U12?U2 construct in which the U12 intron has been introduced between exons no. 5 and no. 6. The CBP20 mRNA level in Wt was taken as 1. Values are shown as the mean ± SD (n = 3) from three independent experiments. (B) Western blot analysis of CBP20 protein levels in transgenic Arabidopsis plants. For each construct, two independent transgenic lines were analyzed. Upper panel – immunoblot using antibodies against CBP20 protein; lower panel – immunoblot with antibodies against actin used as a loading control. Numbers below the western blot image are relative intensities of CBP20 bands calculated using the wt transgene (line 1) CBP20 level as 1. Lines are described as previously.

  • Background
  • Background:

    CBP20 (Nuclear cap-binding protein subunit 2)is a component of the cap-binding complex (CBC), involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). Alternative names: 20 kDa nuclear cap-binding protein,  NCBP 20 kDa subunit

  • Product Citations
  • Selected references: Raczynska et al. (2013). TheSERRATEprotein isinvolved inalternativesplicing inArabidopsis thaliana. Nucleic Acids Res. Oct 16.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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AS09 531 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, B.oleracea, P.endiviifolia

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AS16 ECL-S | extreme low femtogram detection  |  Limited stock

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

Choose the appropriate volume in the drop down menu to the right

From 17 €