CBP20 | nuclear cap-binding protein subunit 2

KLH-conjugated peptide, derived with Arabidopsis thaliana CBP20 protein Q9xFD1, At5g44200

29.6 | 30 kDa

Application example

25 µg of total protein extratcs from 10 days old seedlings of Arabidopsis thaliana  (wild type and a CBP20 mutant) were separated on 12.5 % SDS-PAGE and blotted 1h to PVDF (tank blotting). Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 30 min.

Courtesy of Dr. Sascha Laubinger, ZMBP, Germany

AS09 531 | Anti/CBP80 | nuclear cap-binding protein subunit 1, rabbit antibodies

microRNA antibodies

antibodies to microRNA targets

Plant protein extraction buffer

Secondary antibodies

CBP20 (Nuclear cap-binding protein subunit 2)is a component of the cap-binding complex (CBC), involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). Alternative names: 20 kDa nuclear cap-binding protein,  NCBP 20 kDa subunit