CBP20 | nuclear cap-binding protein subunit 2

AS09 530 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated peptide, derived with Arabidopsis thaliana CBP20 protein Q9xFD1, At5g44200

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 200 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

29.6 | 30 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Glycne max, Hordeum vulgare, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Zea mays

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

25 µg of total protein extratcs from 10 days old seedlings of Arabidopsis thaliana (wild type and a CBP20 mutant) were separated on 12.5 % SDS-PAGE and blotted 1h to PVDF (tank blotting). Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 30 min.

Courtesy of Dr. Sascha Laubinger, ZMBP, Germany

Additional information

Related products

AS09 531 | Anti/CBP80 | nuclear cap-binding protein subunit 1, rabbit antibodies

microRNA antibodies

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Plant protein extraction buffer

Secondary antibodies

Background

CBP20 (Nuclear cap-binding protein subunit 2)is a component of the cap-binding complex (CBC), involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). Alternative names: 20 kDa nuclear cap-binding protein, NCBP 20 kDa subunit