CDJ1 | chloroplast DnaJ homolog 1

Name: CDJ1 | chloroplast DnaJ homolog 1
SKU: AS06 179
Price: 394 EUR
Availability: InStock

AS06 179 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Chlamydomonas reinhardtii

CDJ1 | chloroplast DnaJ homolog 1 in the group Antibodies Plant/Algal / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS06 179)

Product Information

Immunogen

fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1, 3000 (WB)

Expected | apparent MW

40 | 40 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity

Ostreococcus sp.

Species of your interest not listed? Contact us

Not reactive in Higher plants

Application examples

Additional information

Western blot has been done on 10-50 µg load per well; immunoprecipitation has been done on 5-50 µl

Background

CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.

Product citations

Selected references Willmund et al (2008). The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C. Plant Physiol. 148: 2070-2082.

Related products: CDJ1 | chloroplast DnaJ homolog 1

Agrisera ECL kit (Bright/SuperBright)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only...

From 26 €

Info

Add to cart

CGE1 | Alfa-CGE1, chloroplastic GrpE homolog

AS06 176 | Clonality: Polyclonal | Host: Rabbit | Reactivity: ...

394 €

Goat anti-Rabbit IgG (H&L), ALP conjugated

AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until...

206 €

Goat anti-Rabbit IgG (H&L), HRP conjugated

AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...

201 €

immunogen:	fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	100 µl
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunoprecipitation (IP), Western blot (WB)
recommended dilution:	1, 3000 (WB)
Expected \| apparent MW:	40 \| 40 kDa
Confirmed reactivity:	Chlamydomonas reinhardtii
predicted reactivity:	Ostreococcus sp. Species of your interest not listed? Contact us
not reactive in:	Higher plants
additional information (application):	Western blot has been done on 10-50 µg load per well; immunoprecipitation has been done on 5-50 µl
background:	CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.
All references:	Willmund et al (2008). The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C. Plant Physiol. 148: 2070-2082.