Chicken anti-Goat IgG (H&L), ALP conjugated

Name: Chicken anti-Goat IgG (H&L), ALP conjugated
SKU: AS10 1112
Price: 233 EUR
Availability: InStock

AS10 1112 | Clonality: Polyclonal | Host: Chicken | Reactivity: Goat IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Chicken anti-Goat IgG (H&L), ALP conjugated in the group Secondary Antibodies / Anti-Goat / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS10 1112)

Product Information

Host Chicken

Clonality Polyclonal

Purity Immunogen affinity purified IgY.

Format Liquid

Quantity 1 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Recommended dilution 1 : 500-1 : 2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Background Chicken anti-goat IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.

Product citations

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Host:	Chicken
Clonality:	Polyclonal
Purity:	Immunogen affinity purified IgY.
Format:	Liquid
Quantity:	1 mg
storage:	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
recommended dilution:	1 : 500-1 : 2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)
additional information:	This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins. Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
background:	Chicken anti-goat IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.