Chicken anti-Goat IgG (H&L), FITC conjugated, min, cross-reactivity to human, mouse,rabbit IgG/serum

Product no: AS10 1259

AS10 1259 | Clonality: Polyclonal  |  Host:Chicken   |  Reactivity: Goat IgG (H&L)

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  • Product Info
  • Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgY.
    Format: Liquid
    Quantity: 0,5 mg
    Storage: Store at 2-8C. Shelf life of this product is one year from date of receipt.
    Recommended dilution: 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (Flow cyt)
  • Additional Information
  • Additional information:

    This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.

    Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

    BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

  • Background
  • Background: Chicken anti-goat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human,mouse,rabbit IgG/serum and are affinity purified using solid phase goat IgG.
  • Protocols
  • Antibody protocols
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