showForm Chicken anti-goat IgG (H&L), FITC conjugated, adsorbed

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Chicken anti-Goat IgG (H&L), FITC conjugated, min. cross-reactivity to human, mouse,rabbit IgG/serum

AS10 1259   | Clonality: Polyclonal  |  Host:Chicken   |  Reactivity:Goat IgG (H&L)

Chicken anti-Goat IgG (H&L), FITC conjugated, min. cross-reactivity to human, mouse,rabbit IgG/serum  in the group Secondary Antibodies / Anti-Goat / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS10 1259)

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Product Information

Host Chicken
Clonality Polyclonal
Purity Affinity purified IgY
Format Liquid
Quantity 0.5 mg
Storage Store at 2-8°C.Shelf life of this product is one year from date of receipt.
Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (Flow cyt)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

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Background

Background Chicken anti-goat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human,mouse,rabbit IgG/serum and are affinity purified using solid phase goat IgG.

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