Chicken anti-Mouse IgG (H&L), ALP conjugated

AS10 1125 | Clonality: Polyclonal | Host:Chicken | Reactivity:Mouse IgG (H&L)


product information
Background		Chicken anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Host		Chicken
Clonality		Polyclonal
Clone
Purity		Affinity purified IgY
Format		Liquid
Quantity		1 mg
Reconstitution
Storage		Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products		other ALP conjugated secondary antibodies to moue immunoglobulins
Additional information		This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins. Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

application information
Recommended dilution		1 : 500-1 : 2000 (WB) 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC),
Expected \| apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references

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