Chicken anti-Rabbit IgG (H&L), HRP conjugated

Product no: AS10 833

AS10 833 | Clonality: Polyclonal  |  Host: Chicken  | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

206
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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Purified Rabbit IgG

    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified chicken IgY.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
    Storage: Store non-diluted antibody at 2-8C. For storage at -20C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: The optimal working dilution should be determined by the investigator
  • Reactivity
  • Confirmed reactivity: Rabbit IgG (H&L)
    Predicted reactivity: Rabbit IgG (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.2)

    HRP-conjugate is supplied in PBS, 1% BSA and 0.1% proclin 150.

    0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

    Additional information (application):

    This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

    No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

  • Background
  • Background:

    Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.

  • Product Citations
  • Selected references: Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.
    Gao et al. (2018). Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2. Plant Biotech. J. 10.1111/pbi.13046
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
  • Reviews:
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AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S

This product can be purchased in 3 different volumes:

AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer

AS16 ECL-SN, 100 ml

AS16 ECL-SN-1L, 1L

Choose the appropriate volume in the drop down menu to the right

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