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Chicken anti-Rat IgG (H&L), DyLightŪ 550 conjugated, min. cross-reactivity to human or rabbit IgG or serum proteins

ASJIMTESTAR | Host: Chicken | Clonality: Polyclonal | Reactivity:
Chicken anti-Rat IgG (H&L), DyLightŪ 550 conjugated, min. cross-reactivity to human or rabbit IgG or serum proteins in the group Secondary Antibodies / Anti-Rat / Fluorescent / DyLightŪ / DyLightŪ 550 at Agrisera AB (Antibodies for research) (AS12 1973)

PRODUCT DATA SHEET

Qty: 
195

Data sheet Product citations Protocols Add review

Product Information

Immunogen

Purified rat IgG

Host Chicken
Clonality Polyclonal
Purity Affinity purified chicken IgG
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications none
Conjugation DyLightŪ 550
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.

No reactivity is observed to: non-immunoglobulin rat serum proteins  IgG from human or rabbit  serum proteins from human or rabbit.

Related products

Background

Background

Chicken anti-Rat IgG (H&L) - DyLight®550 Conjugate, min. cross-reactivity to human or rabbit IgG or serum proteins is a secondary antibody conjugated to  DyLight® 550, which binds to all rat IgG (H&L) in immunological assays. 

DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

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