Chicken anti-Rat IgG (H&L), DyLight® 550 conjugated, min, cross-reactivity to human or rabbit IgG or serum proteins

Product no: AS12 1973

AS12 1973 | Clonality: Polyclonal  |  Host: Chicken | Reactivity: Rat IgG

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  • Product Info
  • Immunogen:

    Purified rat IgG

    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified chicken IgG.
    Format: Lyophilized
    Quantity: 0.5 mg
    Reconstitution: For reconstitution add 0.55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Tested applications: none
    Recommended dilution: 1 : 20-1 : 2000 for most applications
  • Additional Information
  • Additional information: Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
    Additional information (application):

    Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.

    No reactivity is observed to: non-immunoglobulin rat serum proteins  IgG from human or rabbit  serum proteins from human or rabbit.

  • Background
  • Background:

    Chicken anti-Rat IgG (H&L) - DyLight®550 Conjugate, min. cross-reactivity to human or rabbit IgG or serum proteins is a secondary antibody conjugated to  DyLight® 550, which binds to all rat IgG (H&L) in immunological assays. 

    DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).

    DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody protocols
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