Chicken anti-Rat IgG (H&L), DyLightŪ 550 conjugated, min. cross-reactivity to human or rabbit IgG or serum proteins
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Purified rat IgG
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.
No reactivity is observed to: non-immunoglobulin rat serum proteins IgG from human or rabbit serum proteins from human or rabbit.
Chicken anti-Rat IgG (H&L) - DyLight®550 Conjugate, min. cross-reactivity to human or rabbit IgG or serum proteins is a secondary antibody conjugated to DyLight® 550, which binds to all rat IgG (H&L) in immunological assays.
DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).
DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.