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ZCP2 | Zinc Chaperone Protein

AS12 1848   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii | zinc deficiency marker

ZCP2 | Zinc Chaperone Protein in the group Antibodies for Plant/Algal  / Environmental Stress / Heavy metal stress at Agrisera AB (Antibodies for research) (AS12 1848)

DATA SHEET IN PDF

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Data sheet Product citations Add review

Product Information

Immunogen

Recombinant ZCP2 protein of Chlamydomonas reinhardtii,  ID 536252, UniProt: A0A059VIF5

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

70 | 100 kDa (probably due to glycosylation)

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Emiliania huxleyi
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-COG0523 Zinc deficiency protein

Chlamydomonas reinhardtii soluble proteins (10 µg) were separated on a 7.5% SDS-PAGE gel and blotted to nitrocellulose for 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was
decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in PBS-T + 1% milk at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech ) diluted to 1:3000 in PBS-T + 1% milk for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T + 1% milk at RT with agitation, then rinsed with TBS (pH 7.5), and developed.

Courtesy Dr. Dudley Page, UCLA, USA

Additional information

This antibody can be used as a marker of zinc homeostasis in Chlamydomonas reinhardtii.

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Background

Background

COG0523 (Zinc defficiency induced protein) is a putative metal chaperone from G3E family of GTPases which acts in metal trafficking. 

Product citations

Selected references Hsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.

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