ZCP1 | Zinc Chaperone Protein

Product no: AS14 2770

AS14 2770  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

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  • Product Info
  • Immunogen:

    Recombinant, full length ZCP1 protein of Chlamydomonas reinhardtii, Cre14.g630871, UniProt: A0A059VIM6

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    35 | 46 kDa

  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii
    Predicted reactivity: Emiliania huxleyi
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

     

    western blot using anti-ZCP1 antibodies

    Chlamydomonas reinhardtii
    whole-cell extracts corresponding to 10 μl 1 x 10 7 cells/ml per well (except dilutions as indicated) were separated on a 12% SDS-PAGE gel and blotted to nitrocellulosefor 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% bovine calf serum in PBS-T overnight at 4deg C. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in PBS-T with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech) diluted to 1:3000 in PBS-T for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T, then rinsed with TBS, and developed.

    Courtesy of Crysten Blaby, UCLA, USA
  • Additional Information
  • Additional information (application): This antibody can be used as a marker of zinc homeostasis in Chlamydomonas reinhardtii.
  • Background
  • Background: ZCP1 belongs to a large family of putative metallochaperones, likned with maintainance of zinc homeostasis in all living organisms.
  • Product Citations
  • Selected references: 38221900
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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