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Anti-ZCP1 | Zinc Chaperone Protein
Product no: AS14 2770
AS14 2770 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
329
€
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3-6 business days
- Product Info
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Immunogen: Recombinant, full length ZCP1 protein of Chlamydomonas reinhardtii, Cre14.g630871, UniProt: A0A059VIM6
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 35 | 46 kDa
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Emiliania huxleyi
Species of your interest not listed? Contact usNot reactive in: Triticum aestivum - Application Examples
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application example
Chlamydomonas reinhardtii whole-cell extracts corresponding to 10 μl 1 x 10 7 cells/ml per well (except dilutions as indicated) were separated on a 12% SDS-PAGE gel and blotted to nitrocellulosefor 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% bovine calf serum in PBS-T overnight at 4deg C. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in PBS-T with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech) diluted to 1:3000 in PBS-T for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T, then rinsed with TBS, and developed.
Courtesy of Crysten Blaby, UCLA, USA - Additional Information
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Additional information (application): This antibody can be used as a marker of zinc homeostasis in Chlamydomonas reinhardtii. - Background
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Background: ZCP1 belongs to a large family of putative metallochaperones, likned with maintainance of zinc homeostasis in all living organisms. - Reviews:
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