ZCP1 | Zinc Chaperone Protein
AS14 2770 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
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Recombinant, full length ZCP1 protein of Chlamydomonas reinhardtii, Cre14.g630871, UniProt: A0A059VIM6
35 | 46 kDa
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Chlamydomonas reinhardtii whole-cell extracts corresponding to 10 μl 1 x 10 7 cells/ml per well (except dilutions as indicated) were separated on a 12% SDS-PAGE gel and blotted to nitrocellulosefor 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% bovine calf serum in PBS-T overnight at 4deg C. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in PBS-T with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech) diluted to 1:3000 in PBS-T for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T, then rinsed with TBS, and developed.
Courtesy of Crysten Blaby, UCLA, USA