ZCP1 | Zinc Chaperone Protein
AS14 2770 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
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Product Information
Immunogen
Recombinant, full length ZCP1 protein of Chlamydomonas reinhardtii, Cre14.g630871, UniProt: A0A059VIM6
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 ĩl
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
35 | 46 kDa
Reactivity
Confirmed reactivity
Chlamydomonas reinhardtii
Predicted reactivity
Emiliania huxleyi
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Chlamydomonas reinhardtii whole-cell extracts corresponding to 10 μl 1 x 10 7 cells/ml per well (except dilutions as indicated) were separated on a 12% SDS-PAGE gel and blotted to nitrocellulosefor 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% bovine calf serum in PBS-T overnight at 4deg C. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in PBS-T with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech) diluted to 1:3000 in PBS-T for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T, then rinsed with TBS, and developed.
Courtesy of Crysten Blaby, UCLA, USA
application example
Chlamydomonas reinhardtii whole-cell extracts corresponding to 10 μl 1 x 10 7 cells/ml per well (except dilutions as indicated) were separated on a 12% SDS-PAGE gel and blotted to nitrocellulosefor 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% bovine calf serum in PBS-T overnight at 4deg C. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in PBS-T with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech) diluted to 1:3000 in PBS-T for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T, then rinsed with TBS, and developed.
Courtesy of Crysten Blaby, UCLA, USA
Additional information
This antibody can be used as a marker of zinc homeostasis in Chlamydomonas reinhardtii.
Background
Background
ZCP1 belongs to a large family of putative metallochaperones, likned with maintainance of zinc homeostasis in all living organisms.
Product citations
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