Name:
Phone:
E-mail:
Message


 


CrPDAT1 | Phospholipid: diacylglycerol acyltransferase

AS12 1875  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

CrPDAT1 | Phospholipid: diacylglycerol acyltransferase in the group Antibodies for Plant/Algal  / Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS12 1875)

DATA SHEET IN PDF

Qty: 
373
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen recombinant CrPDAT1 without transmembrane domains, overexpressed in E.coli,
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 350 ĩl
Reconstitution For reconstitution add 350 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 250 (WB) load per well up to 30 ug
Expected | apparent MW

140 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Chlamydomonas reinhardtii
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-PADT antibody

Total proteins (containing 2.5 to 30 ug ) from Chlamydomonas cells extracted with lysis buffer (50 mM Tris-HCl, pH 6.8, containing 2% SDS and 10 mM EDTA and a protease inhibitor cocktail) were separated on 10 % SDS-PAGE and transferred onto a nitrocellose blot over night at 4°C. Blots were blocked with blocking buffer ( 5% (w/v) non-fat dry milk powder in TBS-T) for 2 hrs at room temperature (RT) with agitation. Blot was incubated in the primary antibody (∆TMCrPDAT) at a dilution of 1:250 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then whashed 5 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Bio-Rad) diluted to 1:5000 in the same buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Chemiluminescence detection kit (Bio-Rad) according to the manufacturers instructions. An imaging system (ChemiDoc XRS; Bio-Rad) was used to quantitatively and qualitatively analyze protein blot. Exposure time was 30 seconds.

Courtesy of Dr. Kangsup Yoon, Arizona State University.

Additional information

Related products

Background

Background PDAT1 is an enzyme phospholipid: diacylglycerol acyltransferase, which catalyzes TAG (triglycerols) synthesis. It has a strong lipase activity with a broad substrate specificity.

Product citations

Selected references Yoon et al (2012). Phospholipid:Diacylglycerol Acyltransferase Is a Multifunctional Enzyme Involved in Membrane Lipid Turnover and Degradation While Synthesizing Triacylglycerol in the Unicellular Green Microalga Chlamydomonas reinhardtii. Plant Cell, Oct 2012.

Related products: CrPDAT1 | Phospholipid: diacylglycerol acyltransferase

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection...
From 15 €
AS10 938 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
...
373 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
...
190 €