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Anti-Cyc1 | Cyclin-B1-1
Product no: AS19 4339
AS19 4339 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
248
€
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Delivery:
7-10 business days
- Product Info
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Immunogen: Recombinant Cyclin-B1-1 protein derived from Arabidopsis thaliana sequence, amino acids: 1-428. UniProt: P30183 TAIR: AT4G37490 Host: Rabbit Clonality: Polyclonal Purity: >95%, Protein G purified to a total immunoglobulin G fraction. Format: Liquid Quantity: 100 µg Storage: Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1:1000-1:5000 (WB) Expected | apparent MW: 48.4 | 49 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Brassica campestris, Capsella rubella, Eutrema salsugineum, Noccaea caerulescens
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example
40 and 20 µg of recombinant Arabidopsis thaliana CYCB1-1 protein were loaded/well and separated on 10 % SDS-PAGE and blotted 1h to PVDF (0.45 µm) using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 1.8 µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions. - Additional Information
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Additional information: Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4
Reactivity of this antibody on endogenous extract remains to be confirmed. - Background
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Background: Cyclin-B1-1 is a cyclin-dependent protein kinase involved in cell division. Functions as an effector of growth contol at G2/M.
Alternative names: Cyc1-At, G2/mitotic-specific cyclin-B1-1, CycB1;1, CYCB1-1, CYC1 - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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