Cyc1 | Cyclin-B1-1

AS19 4339 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen Recombinant Cyclin-B1-1 protein derived from Arabidopsis thaliana sequence, amino acids: 1-428. UniProt: P30183 TAIR: AT4G37490

Host Rabbit

Clonality Polyclonal

Purity >95%, Protein G purified to a total immunoglobulin G fraction.

Format Liquid

Quantity 100 µg

Storage Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1:1000-1:5000 (WB)

Expected | apparent MW 48,4 | 49 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Brassica campestris, Capsella rubella, Eutrema salsugineum, Noccaea caerulescens
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

Western blot using anti-plant CYCB1 antibodies

40 and 20 µg of recombinant Arabidopsis thaliana CYCB1-1 protein were loaded/well and separated on 10 % SDS-PAGE and blotted 1h to PVDF (0.45 µm) using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 1.8 µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions.

Additional information

Additional information Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4

Reactivity of this antibody on endogenous extract remains to be confirmed.

Background

Background Cyclin-B1-1 is a cyclin-dependent protein kinase involved in cell division. Functions as an effector of growth contol at G2/M.
Alternative names: Cyc1-At, G2/mitotic-specific cyclin-B1-1, CycB1;1, CYCB1-1, CYC1

Product citations

Picture (footer):	application example 40 and 20 µg of recombinant Arabidopsis thaliana CYCB1-1 protein were loaded/well and separated on 10 % SDS-PAGE and blotted 1h to PVDF (0.45 µm) using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 1.8 µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions.
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Brassica campestris, Capsella rubella, Eutrema salsugineum, Noccaea caerulescens Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4 Reactivity of this antibody on endogenous extract remains to be confirmed.
background:	Cyclin-B1-1 is a cyclin-dependent protein kinase involved in cell division. Functions as an effector of growth contol at G2/M. Alternative names: Cyc1-At, G2/mitotic-specific cyclin-B1-1, CycB1;1, CYCB1-1, CYC1
calculated \| apparent molecular mass [kDa]:	48,4 \| 49 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	Recombinant Cyclin-B1-1 protein derived from Arabidopsis thaliana sequence, amino acids: 1-428. UniProt: P30183 TAIR: AT4G37490
Purity:	>95%, Protein G purified to a total immunoglobulin G fraction.
Quantity:	100 µg
recommended dilution:	1:1000-1:5000 (WB)
storage:	Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)