Cyc1 | Cyclin-B1-1
AS19 4339 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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40 and 20 µg of recombinant Arabidopsis thaliana CYCB1-1 protein were loaded/well and separated on 10 % SDS-PAGE and blotted 1h to PVDF (0.45 µm) using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 1.8 µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions.
Reactivity of this antibody on endogenous extract remains to be confirmed.
Alternative names: Cyc1-At, G2/mitotic-specific cyclin-B1-1, CycB1;1, CYCB1-1, CYC1
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