ARC5 | Dynamin-like protein ARC5

AS13 2676 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from internal domain of Dynamin-like protein ARC5 from Arabidopsis thaliana Uniprot: Q84N64,TAIR: AT3G19720

Host Rabbit

Clonality Polyclonal

Purity Total IgG

Format Lyophilized in Tris 0,1M, glycine 0,1M, sucrose 2%

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Shelf life upon re-constitution is 6 months. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Store reconstituted antibodies at 4°C or in -20° for extrended periods of time.Lyophilized powder is stable for a minimum of 2 years at -20°C.

Tested applications Western blot (WB)

Recommended dilution 1 : 500-1 : 5 000 (WB)

Expected | apparent MW

87 kDa

Reactivity

Predicted reactivity Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Antibody format is a total IgG (purified on Protein A).

Related products

collection of antibodies to proteins involved in photosynthesis

Plant protein extraction buffer

Secondary antibodies

Background

ARC5 (Dynamin-like protein ARC5) is a probable GTPase component of both plastid and peroxisme division machinery. Required for the last steps of plastid division specifically in mesophyll-cell, when the narrow isthmus breaks, facilitating the separation of the daughter plastids. Necessary for peroxisome activities. Seems to influence stromule (stroma-filled tubular extensions of the plastid envelope membrane) length and frequency.

Alternative names: Dynamin-related protein 5B Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5.

Product citations

Selected references Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.