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AGO set
Tag Antibody Set 

CURT1A | Curvature thylakoid 1A

AS08 316  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

CURT1A | Curvature thylakoid 1A in the group Antibodies for Plant/Algal  / Photosynthesis  / Light acclimation at Agrisera AB (Antibodies for research) (AS08 316)

PRODUCT INFORMATION IN PDF

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Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CURT1A sequence, TAIR: AT4G01150

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Blue Native PAGE (2D BN-PAGE), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

17.6 | 11 kDa (due to N-terminal processing)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Phaseolus coccineus
Predicted reactivity Nicotiana tabacum, Zea mays
Not reactive in

Hordeum vulgare

Application examples

Application examples

application example

western blot using anti-Curt1A antibodies

The dillution series of  Hordeum vulgare (4; 2; 1 µg Chl) and Arabidopsis thaliana (4; 2; 1 µg Chl) thylakois were separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 min.

Courtesy of Dr. Marta Powikrowska, University of Copenhagen, Danmark

Additional information

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Background

Background

Curvature thylakoid 1A (CURT1A) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D.  It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.

Product citations

Selected references Armbruster et al. (2013). Arabidopsis CURVATURE THYLAKOID1 Proteins Modify Thylakoid Architecture by Inducing Membrane Curvature. 2013 Jul;25(7):2661-78. doi: 10.1105/tpc.113.113118. Epub 2013 Jul 9.