CURT1A | Curvature thylakoid 1A

PRODUCT INFORMATION IN PDF

product information Background Curvature thylakoid 1A (CURT1A) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D.  It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins. Immunogen KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CURT1A sequence, TAIR: AT4G01150 Host Rabbit Clonality Polyclonal Clone Purity Affinity purified serum in PBS, pH 7.4 Format Lyophilized Quantity 200 µg Reconstitution For reconstitution add 200 µl of sterile water. Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications Blue Native PAGE (2D BN-PAGE), Western blot (WB) Related products photosynthetic antibody collection Plant protein extraction buffer Secondary antibodies Additional information

application information
Recommended dilution		1 : 1000 (WB)
Expected | apparent MW		17.6 | 11 kDa (due to N-terminal processing)
Confirmed reactivity		Arabidopsis thaliana, Phaseolus coccineus
Predicted reactivity		Nicotiana tabacum, Zea mays
Not reactive in		Hordeum vulgare
Additional information
Selected references		Armbruster et al. (2013). Arabidopsis CURVATURE THYLAKOID1 Proteins Modify Thylakoid Architecture by Inducing Membrane Curvature. 2013 Jul;25(7):2661-78. doi: 10.1105/tpc.113.113118. Epub 2013 Jul 9.

application example

The dillution series of  Hordeum vulgare (4; 2; 1 µg Chl) and Arabidopsis thaliana (4; 2; 1 µg Chl) thylakois were separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 min.

Courtesy of Dr. Marta Powikrowska, University of Copenhagen, Danmark

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