CURT1A | Curvature thylakoid 1A
AS08 316 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Pisum sativum
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Hordeum vulgare, Phaseolus coccineus
Courtesy of Dr. Marta Powikrowska, University of Copenhagen, Danmark
Plant species: Arabidopsis LD – Arabidopsis thaliana grown under long day conditions Arabidopsis SD – Arabidopsis thaliana grown under short day conditions Runner bean – Phaseolus coccineus L. Pea – Pisum sativum L. Experimental procedure: Samples of isolated thylakoids containing 50 µg of chlorophyll were denatured with 150 mL double diluted Roti®-Load 1 (ROTH, Art.-Nr. K929.1) at 95 °C for 2 min. Denatured samples containing 0.5 and 1 µg of chlorophyll were loaded in the gel wells and separated on 14% SDS-PAGE gels and blotted for 45 min at 105 V to PVDF membrane using wet transfer. Blot was blocked with 5% Amersham™ ECL Prime Blocking Agent (cat no: RPN418) in TBS-T for 30 min at room temperature (RT) with agitation. The blot was incubated with the primary antibody at a dilution of 1:1000 overnight at 4˚C with agitation. The antibody solution was decanted and the blot was washed 2 times for 5 min in TBS-T at RT with agitation. The blot was incubated using a secondary antibody (goat anti-rabbit IgG HRP conjugated, from Agrisera, AS09 602) diluted to 1: 25000 in 1% Blocking Agent in TBS-T for 1h at RT with agitation. The blot was washed 2 times for 5 min in TBS-T and developed for 5 min with chemiluminescent reagent, according to instructions. Exposure time was 12 minutes in C-Digit chemiluminescence scanner (Li-COR).
Dr Radosław Mazur, Faculty of Biology, University of Warsaw, Poland
Curvature thylakoid 1A (CURT1A) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D. It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.
Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.
Liang et al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.
Armbruster et al. (2013). Arabidopsis CURVATURE THYLAKOID1 Proteins Modify Thylakoid Architecture by Inducing Membrane Curvature. 2013 Jul;25(7):2661-78. doi: 10.1105/tpc.113.113118. Epub 2013 Jul 9.
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