CURT1B | Curvature thylakoid 1B

Product no: AS19 4289

AS19 4289   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CURT1B sequence, UniProt: Q8LCA1, TAIR: At2g46820

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000 (WB)
    Expected | apparent MW: 18,48 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Nicotiana tabacum, Zea mays

    Species of your interest not listed? Contact us
  • Application Examples
  • Application example

    Western blot using anti-CURT1B antibodies

    Samples with 2 µg of total chlorophyll from Arabidopsis thaliana thylakoid fractions were denatured with 250mM DTT at 95°C 5 min, extracts were separated on 12% SDS-PAGE and blotted 7 min to PVDF using semi-dry transfer. Blot was incubated in the primary antibody (and 5% milk) at a dilution of 1: 2000 ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed with Agrisera chemiluminescent detection reagent, ECLSuperBright. Exposure time was 5 seconds.

    Courtesy of Guillem Borràs I Gas , Pribil Lab, University of Copenhagen, Danmark

  • Background
  • Background:

    Curvature thylakoid 1B (CURT1B) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D.  It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.

    Alternative names: Photosystem I protein P, Thylakoid membrane phosphoprotein 14 kDa, PSAP
  • Protocols

  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela

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AS19 4287   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

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AS08 316  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Pisum sativum

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AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 16 €
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