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CURT1B | Curvature thylakoid 1B

AS19 4289   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

CURT1B | Curvature thylakoid 1B in the group Antibodies Plant/Algal  / Photosynthesis  / Biogenesis/architecture at Agrisera AB (Antibodies for research) (AS19 4289)
CURT1B | Curvature thylakoid 1B



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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CURT1B sequence, UniProt: Q8LCA1, TAIR: At2g46820

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW 18,48 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Nicotiana tabacum, Zea mays

Species of your interest not listed? Contact us

Application examples

Application examples Application example

Western blot using anti-CURT1B antibodies

Samples with 2 µg of total chlorophyll from Arabidopsis thaliana thylakoid fractions were denatured with 250mM DTT at 95°C 5 min, extracts were separated on 12% SDS-PAGE and blotted 7 min to PVDF using semi-dry transfer. Blot was incubated in the primary antibody (and 5% milk) at a dilution of 1: 2000 ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed with Agrisera chemiluminescent detection reagent, ECLSuperBright. Exposure time was 5 seconds.

Courtesy of Guillem Borràs I Gas , Pribil Lab, University of Copenhagen, Danmark

Additional information

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Background

Background

Curvature thylakoid 1B (CURT1B) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D.  It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.

Alternative names: Photosystem I protein P, Thylakoid membrane phosphoprotein 14 kDa, PSAP

Product citations

background:

Curvature thylakoid 1B (CURT1B) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D.  It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.

Alternative names: Photosystem I protein P, Thylakoid membrane phosphoprotein 14 kDa, PSAP
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Nicotiana tabacum, Zea mays

Species of your interest not listed? Contact us
calculated | apparent molecular mass [kDa]: 18,48 kDa
Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CURT1B sequence, UniProt: Q8LCA1, TAIR: At2g46820

Purity: Immunogen affinity purified serum in PBS pH 7.4.
Quantity: 50 ĩg
recommended dilution: 1 : 2000 (WB)
Reconstitution: For reconstitution add 50 ĩl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
Picture (footer): Application example

Western blot using anti-CURT1B antibodies

Samples with 2 µg of total chlorophyll from Arabidopsis thaliana thylakoid fractions were denatured with 250mM DTT at 95°C 5 min, extracts were separated on 12% SDS-PAGE and blotted 7 min to PVDF using semi-dry transfer. Blot was incubated in the primary antibody (and 5% milk) at a dilution of 1: 2000 ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed with Agrisera chemiluminescent detection reagent, ECLSuperBright. Exposure time was 5 seconds.

Courtesy of Guillem Borràs I Gas , Pribil Lab, University of Copenhagen, Danmark

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