DCL3 | Dicer-like protein 3
AS12 2103 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL3 sequence, Uniprot: Q9LXW7 , TAIR: At3g43920
117 kDa
Reactivity
Zea mays
Application examples
application example
50 µg of total protein from Arabidopsis thaliana total cell extract which came from the ground, frozen powder and has been directly transferred to 2x Laemmli sample buffer was separated on 15 % SDS-PAGE and blotted over night at 64 mA to nitrocellulose (wet transfer). Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 (1) and 1: 500 (2) 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 1 min.
Courtesy of Dr. Sascha Laubinger, ZMBP, Germany
Additional information
Recommend protein load is from 40-50 µg/well (using 1.5 mm spacers helps to obtain wider wells)
Background
DCL3 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs).
Synonymes: Endoribonuclease Dicer homolog 3.
Product citations
Related products: DCL3 | Dicer-like protein 3
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