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DCL3 | Dicer-like protein 3

AS12 2103 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

DCL3 | Dicer-like protein 3 in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / microRNA at Agrisera AB (Antibodies for research) (AS12 2103)

DATA SHEET IN PDF

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL3 sequence, Uniprot: Q9LXW7 , TAIR: At3g43920

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

117 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabidopsis thaliana
Not reactive in

Zea mays

Application examples

Application examples

application example

western blot using anti-DCL3 antibodies

50 µg of total protein from Arabidopsis thaliana total cell extract which came from the ground, frozen powder and has been directly transferred to 2x Laemmli sample buffer was separated on 15 % SDS-PAGE and blotted over night at 64 mA to nitrocellulose (wet transfer). Blots were blocked with Roti-block over night at 4°C agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 (1) and 1: 500 (2) 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 1 min.


Courtesy of Dr. Sascha Laubinger, ZMBP, Germany

Additional information

Recommend protein load is from 40-50 µg/well (using 1.5 mm spacers helps to obtain wider wells)

Related products

Related products

AS19 4307 | anti-DCL1 | Dicer-like protein 1, rabbit antibodies
AS15 3100 | anti-DCL2 | Endoribonuclease Dicer homolog 2, rabbit antibodies
AS15 3105 | anti-DCL4 | Dicer-like protein 4, rabbit antibodies

collection of antibodies to proteins involved in microRNA metabolism

Plant protein extraction buffer

Secondary antibodies

Background

Background

DCL3 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs).

Synonymes: Endoribonuclease Dicer homolog 3.

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