Deg8 | protease Do-like 8 (chloroplastic)

Name: Deg8 | protease Do-like 8 (chloroplastic)
SKU: AS14 2767
Price: 302 EUR
Availability: InStock

AS14 2767 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabacum

Deg8 | protease Do-like 8 (chloroplastic) in the group Antibodies Plant/Algal / Photosynthesis / Proteases at Agrisera AB (Antibodies for research) (AS14 2767)

Deg8 | protease Do-like 8 (chloroplastic)

Product Information

Immunogen

Recombinant mature Deg8 of Arabidopsis thaliana UniProt: Q9LU10,TAIR:AT5G39830, overexpressed in E.coli

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

47 | 38 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabacum

Predicted reactivity Brassica pekinensis, Glycine max, Phaseolus vulgaris, Populus balsamifera, Ricinus communis, Solanum lycopersicum, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using anti-Deg8 antibodies

Total protein from Arabidopsis thaliana was extracted with buffer containing 0.2M tris-HCl pH 6.8, 10% Glycerol, 8% SDS, 20% β-mercaptoethanol, 5M Urea. 10 µg of chlorophyll were separated on 12 % SDS-PAGE using tank transfer and blotted 1.5h to NC. Blots were blocked with 5% nonfat dry milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in 5% nonfat dry milk in TBS-T over night at 40C with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed once for 5 min and 2 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated from Sigma) diluted to 1:10000 in 5% nonfat dry milk in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer instructions. Exposure time was 1 minute.

Courtesy of Leah Naveh, The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Israel

Additional information

Primary antibody can be also incubated using 1 hour at RT protocol

Background

Deg8 protease forms a hexamer together with Deg5 in the thylakoid lumen and is involved in the cleavage of photodamaged D2 protein of photosystem II. Localized in thylakoid lumen.

Product citations

immunogen:	Recombinant mature Deg8 of Arabidopsis thaliana UniProt: Q9LU10,TAIR:AT5G39830, overexpressed in E.coli
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	47 \| 38 kDa
Confirmed reactivity:	Arabidopsis thaliana, Nicotiana tabacum
predicted reactivity:	Brassica pekinensis, Glycine max, Phaseolus vulgaris, Populus balsamifera, Ricinus communis, Solanum lycopersicum, Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example Total protein from Arabidopsis thaliana was extracted with buffer containing 0.2M tris-HCl pH 6.8, 10% Glycerol, 8% SDS, 20% β-mercaptoethanol, 5M Urea. 10 µg of chlorophyll were separated on 12 % SDS-PAGE using tank transfer and blotted 1.5h to NC. Blots were blocked with 5% nonfat dry milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in 5% nonfat dry milk in TBS-T over night at 40C with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed once for 5 min and 2 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated from Sigma) diluted to 1:10000 in 5% nonfat dry milk in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer instructions. Exposure time was 1 minute. Courtesy of Leah Naveh, The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Israel
additional information (application):	Primary antibody can be also incubated using 1 hour at RT protocol
background:	Deg8 protease forms a hexamer together with Deg5 in the thylakoid lumen and is involved in the cleavage of photodamaged D2 protein of photosystem II. Localized in thylakoid lumen.