Deg8 | protease Do-like 8 (chloroplastic)

Product no: AS14 2767

AS14 2767 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabacum

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  • Product Info
  • Immunogen:

    Recombinant mature Deg8 of Arabidopsis thaliana UniProt: Q9LU10,TAIR:AT5G39830, overexpressed in E.coli

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    47 | 38 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Nicotiana tabacum
    Predicted reactivity: Brassica pekinensis, Glycine max, Phaseolus vulgaris, Populus balsamifera, Ricinus communis, Solanum lycopersicum, Vitis vinifera
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot using anti-Deg8 antibodies
    Total protein from Arabidopsis thaliana was extracted with buffer containing 0.2M tris-HCl pH 6.8, 10% Glycerol, 8% SDS, 20% β-mercaptoethanol, 5M Urea. 10 µg of chlorophyll were separated on 12 % SDS-PAGE using tank transfer and blotted 1.5h to NC. Blots were blocked with 5% nonfat dry milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in 5% nonfat dry milk in TBS-T over night at 40C with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed once for 5 min and 2 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated from Sigma) diluted to 1:10000 in 5% nonfat dry milk in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer instructions. Exposure time was 1 minute.

    Courtesy of Leah Naveh, The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Israel
  • Additional Information
  • Additional information (application): Primary antibody can be also incubated using 1 hour at RT protocol
  • Background
  • Background:

    Deg8 protease forms a hexamer together with Deg5 in the thylakoid lumen and is involved in the cleavage of photodamaged D2 protein of photosystem II. Localized in thylakoid lumen. 

  • Product Citations
  • Selected references: 38221900
  • Protocols
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