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Donkey anti-Mouse IgG (H&L), ALP conjugated, min. cross-reactivity to bovine,chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG

AS10 1110 | Clonality: Polyclonal  |  Host: Donkey Reactivity:Mouse IgG (H&L)

Donkey anti-Mouse IgG (H&L), ALP conjugated, min. cross-reactivity to bovine,chicken, goat, guinea pig, hamster, horse, human,  rabbit, rat or sheep IgG  in the group Secondary Antibodies / Anti-Mouse / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS10 1110)

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Product Information

Host Donkey
Clonality Polyclonal
Purity Affinity purified IgG
Format Liquid
Quantity 1 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

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Background

Background Donkey anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which reactis to all mouse IgGs in immunological assays. Antibody is adsorbed against bovine,chicken,goat, guinea pig,hamster,horse,human,rabbit,rat,sheep IgG and affinity purified using solid phase mouse IgG.

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