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Donkey anti-Mouse IgG (H&L), DyLightŪ 594 conjugated

AS12 2048 | Clonality: Polyclonal  |  Host: Donkey  | Reactivity: Mouse IgG (H&L)

Donkey anti-Mouse IgG (H&L), DyLightŪ 594 conjugated in the group Secondary Antibodies / Anti-Mouse / Fluorescent / DyLightŪ / DyLightŪ 594 at Agrisera AB (Antibodies for research) (AS12 2048)



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Product Information

Immunogen

Purified mouse IgG, whole molecule

Host Donkey
Clonality Polyclonal
Purity Immunogen affinity purified donkey IgG.
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Additional information Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative

Based on  immunoelectrophoresis, this antibody reacts with:  heavy (γ) chains on mouse IgG  light chains on all mouse immunoglobulins.

No reactivity is observed to: non-immunoglobulin mouse serum proteins.

Related products

Background

Background

Donkey anti-Mouse IgG (H&L) , DyLight®594 Conjugate is a secondary antibody conjugated to  DyLight® 594, which binds to all mouse IgG (H&L) in immunological assays. 

DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

additional information: Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
additional information (application):

Based on  immunoelectrophoresis, this antibody reacts with:  heavy (γ) chains on mouse IgG  light chains on all mouse immunoglobulins.

No reactivity is observed to: non-immunoglobulin mouse serum proteins.

Clonality: Polyclonal
Format: Lyophilized
Host: Donkey
immunogen:

Purified mouse IgG, whole molecule

Purity: Immunogen affinity purified donkey IgG.
Quantity: 1 mg
recommended dilution: 1 : 20-1 : 2000 for most applications
Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
background:

Donkey anti-Mouse IgG (H&L) , DyLight®594 Conjugate is a secondary antibody conjugated to  DyLight® 594, which binds to all mouse IgG (H&L) in immunological assays. 

DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

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