Donkey anti-Mouse IgG (H&L), F(ab)'2 fragment, DyLightŪ 633 conjugated
AS12 2182 | Clonality: Polyclonal | Host: Donkey | Reactivity: Mouse IgG

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Product Information
Immunogen
Purified mouse IgG, whole molecule
Host
Donkey
Clonality
Polyclonal
Purity
Immunogen affinity purified donkey IgG.
Format
Lyophilized
Quantity
0,5 mg
Reconstitution
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution
1 : 20-1 : 2000 for most applications
Reactivity
Application examples
Additional information
Additional information
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Background
Background
Donkey anti-Mouse IgG (H&L) - F(ab)'2 fragment, DyLight® 633 Conjugated is a secondary antibody conjugated to DyLight® 633, which binds to mouse IgG (H&L), F(ab)'2 fragment in immunological assays.
DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase mouse IgG.
DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product citations
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