Donkey anti-Mouse IgG (H&L), F(ab)'2 fragment, DyLight® 800 conjugated

AS12 2428 | Clonality: Polyclonal | Host: Donkey | Reactivity: Mouse IgG (H&L) - F(ab)'2 fragment

Product Information

Immunogen

Purified Mouse IgG, whole molecule

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified donkey IgG.

Format Lyophilized

Quantity 0,5 mg

Reconstitution For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Not reactive in

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulins

No reactivity is observed to: non-immunoglobulin mouse serum proteins

Background

Donkey anti-Mouse IgG (H&L) - F(ab)'2 fragment, DyLight®800 Conjugated is a secondary antibody conjugated to DyLight® 800, which binds to Mouse IgG (H&L) - F(ab)'2 fragment in immunological assays.

DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Mouse IgG (H&L)

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

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