Donkey anti-Mouse IgG (H&L), FITC conjugated, min, cross-reactivity to bovine,chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG

AS10 1177 | Clonality: Polyclonal | Host: Donkey | Reactivity: Mouse IgG (H&L)

Product Information

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified IgG.

Format Liquid

Quantity 1 mg

Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.

Recommended dilution 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins and bovine,chicken,goat,guinea pig,hamster,horse, human,rabbit,rat,sheep IgG based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

Background

Background Donkey anti-mouse IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to all mouse IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,rabbit,rat,sheep IgG and affinity purified using solid phase mouse IgG.

Product citations

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