Donkey anti-Rabbit IgG (H&L), DyLight® 405 conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep IgG

AS16 3511 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG

Product Information

Host Donkey

Clonality Polyclonal

Purity Affinity purified

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Storage Store freeze-dried powder at 2-8 °C.

Tested applications Flow cytometry (Flow cyt), Immunolocalization (IL)

Recommended dilution 1 : 20-1 : 2000 (Flow cyt), (IL)

Reactivity

Confirmed reactivity Rabbit IgG (H&L)

Application examples

Additional information

Antibody purity is > 95% based on SDS-PAGE.
Affinity purified using solid phase Rabbit IgG .
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.

Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG • light chains on all rabbit immunoglobulins .
Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep .

Related products

Related products other anti-rabbit secondary antibodies

Background

Background Donkey anti-Rabbit IgG (H&L), DyLight® 405 conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight® 405, which binds to rabbit IgG in immunological assays.
DyLight® 405 has Amax = 400 nm, Emax = 420 nm.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries

Product citations

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