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Goat anti-Rabbit IgG (H&L), DyLightŪ 550 conjugated, min. cross-reactivity to human serum proteins

AS16 3516 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG
Goat anti-Rabbit IgG (H&L), DyLightŪ 550 conjugated, min. cross-reactivity to human serum proteins in the group Secondary Antibodies / Anti-Rabbit / Fluorescent / DyLightŪ / DyLightŪ 550 at Agrisera AB (Antibodies for research) (AS16 3516)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store freeze-dried powder at 2-8 °C.
Tested applications Flow cytometry (Flow cyt), Immunolocalization (IL)
Recommended dilution 1 : 20-1 : 2000 (Flow cyt), (IL)

Reactivity

Confirmed reactivity Rabbit IgG (H&L)

Application examples

Additional information

Additional information

Antibody purity is > 95% based on SDS-PAGE.
Affinity purified using solid phase Rabbit IgG .
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.

Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG • light chains on all rabbit immunoglobulins .
Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • human serum proteins .

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Background

Background

Goat anti-Rabbit IgG (H&L), DyLight® 550 conjugated, min. cross-reactivity to human serum proteins is a secondary antibody conjugated to  DyLight® 550, which binds to rabbit IgG in immunological assays. 
DyLight® 550 has Amax = 562 nm, Emax = 576 nm.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

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