Donkey anti-Rabbit IgG (H&L), FITC conjugated, min, cross-reactivity to mouse IgG/serum

Product no: AS10 1047

AS10 1047 | Clonality: Polyclonal  |  Host: Donkey  |  Reactivity: Rabbit IgG (H&L)

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  • Product Info
  • Host: Donkey
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgG.
    Format: Liquid
    Quantity: 2 mg
    Storage: Store at 2-8C. Shelf life of this product is one year from date of receipt.
    Recommended dilution: 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)
  • Additional Information
  • Additional information:

    This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, mouse IgG or mouse serum based on immunoelectrophoresis.

    Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

  • Background
  • Background:

    Donkey anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to all rabbit IgGs in immunological assays.  FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against mouse IgG/serum and affinity purified using solid phase rabbit IgG.

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