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Donkey anti-Rabbit IgG (H&L), FITC conjugated, min. cross-reactivity to mouse IgG/serum

AS10 1047  | Clonality: Polyclonal  |  Host: Donkey  |  Reactivity: Rabbit IgG (H&L)

Donkey anti-Rabbit IgG (H&L), FITC conjugated, min. cross-reactivity to mouse IgG/serum  in the group Secondary Antibodies / Anti-Rabbit / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS10 1047)

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Product Information

Host Donkey
Clonality Polyclonal
Purity Affinity purified IgG
Format Liquid
Quantity 2 mg
Storage Store at 2-8°C.Shelf life of this product is one year from date of receipt.
Recommended dilution 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, mouse IgG or mouse serum based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

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Background

Background

Donkey anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to all rabbit IgGs in immunological assays.  FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against mouse IgG/serum and affinity purified using solid phase rabbit IgG.

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