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Donkey anti-Rabbit IgG (H&L), HRP conjugated

AS10 845 | Clonality: Polyclonal  |  Host: doneky  | Reactivity: Rabbit IgG (H&L)

Donkey anti-Rabbit IgG (H&L), HRP conjugated in the group Human/Animal Antibodies / Secondary Antibodies/Blocking / Anti-rabbit  / HRP (horseradish peroxidase) at Agrisera AB (Antibodies for research) (AS10 845)

PRODUCT INFORMATION IN PDF

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product information
Background

Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.

Immunogen

Purified Rabbit IgG

Host Donkey
Clonality Polyclonal
Clone
Purity Affinity purified donkey IgG
Format Liquid
Quantity 1 mg
Reconstitution
Storage Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Related products

AS09 602 | Goat anti-rabbit IgG (H&L) HRP conjugated

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

application information
Recommended dilution The optimal working dilution should be determined by the investigator.
Expected | apparent MW
Confirmed reactivity Rabbit IgG (H&L)
Predicted reactivity Rabbit IgG (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

Selected references

to be added when available



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