Donkey anti-Rabbit IgG (H&L), HRP conjugated

AS10 845 | Clonality: Polyclonal | Host: doneky | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Product Information

Immunogen

Purified Rabbit IgG

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified donkey IgG.

Format Liquid

Quantity 1 mg

Storage Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution The optimal working dilution should be determined by the investigator

Reactivity

Confirmed reactivity Rabbit IgG (H&L)

Predicted reactivity Rabbit IgG (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

Background

Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.

Product citations

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additional information:	HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
additional information (application):	This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
background:	Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Confirmed reactivity:	Rabbit IgG (H&L)
predicted reactivity:	Rabbit IgG (H&L)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Clonality:	Polyclonal
Format:	Liquid
Host:	Donkey
immunogen:	Purified Rabbit IgG
Purity:	Immunogen affinity purified donkey IgG.
Quantity:	1 mg
recommended dilution:	The optimal working dilution should be determined by the investigator
storage:	Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)