Donkey anti-Rat IgG (H&L), FITC conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG

AS10 1282 | Clonality: Polyclonal | Host:Donkey | Reactivity: Rat IgG (H&L)

Product Information

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified IgG.

Format Liquid

Quantity 1 mg

Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.

Recommended dilution 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

Background

Background Donkey anti-rat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse, rabbi,sheep IgG and affinity purified using solid phase rat IgG.

Product citations

Selected references Wijnker et al. (2019). The Cdk1/Cdk2 homolog CDKA;1 controls the recombination landscape in Arabidopsis. Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12534-12539. doi: 10.1073/pnas.1820753116.

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Clonality:	Polyclonal
Format:	Liquid
Host:	Donkey
Purity:	Immunogen affinity purified IgG.
Quantity:	1 mg
recommended dilution:	1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)
storage:	Store at 2-8°C. Shelf life of this product is one year from date of receipt.
background:	Donkey anti-rat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse, rabbi,sheep IgG and affinity purified using solid phase rat IgG.
All references:	Wijnker et al. (2019). The Cdk1/Cdk2 homolog CDKA;1 controls the recombination landscape in Arabidopsis. Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12534-12539. doi: 10.1073/pnas.1820753116.
additional information:	This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep based on immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.