Donkey anti-Sheep IgG (H&L), DyLightŪ 550 conjugated, min. cross reactivity to human or rabbit IgG
AS12 1984 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG
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Product Information
Purified Sheep IgG, whole molecule
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Reactivity
Application examples
Additional information
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins.
No reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human or rabbit.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Background
Donkey anti-Sheep IgG (H&L) - DyLight®550 Conjugated, min. cross reactivity to human or rabbit IgG is a secondary antibody conjugated to DyLight® 550, which binds to all Sheep IgG (H&L) in immunological assays.
DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase Sheep IgG (H&L).
DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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