Donkey anti-Sheep IgG (H&L), DyLight® 550 conjugated, min, cross reactivity to human or rabbit IgG

AS12 1984 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG

Product Information

Immunogen

Purified Sheep IgG, whole molecule

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified donkey IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Additional information Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins.

No reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human or rabbit.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Related products

other dylight anti-sheep secondary antibodies

Background

Donkey anti-Sheep IgG (H&L) - DyLight®550 Conjugated, min. cross reactivity to human or rabbit IgG is a secondary antibody conjugated to DyLight® 550, which binds to all Sheep IgG (H&L) in immunological assays.

DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase Sheep IgG (H&L).

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

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