Donkey anti-Sheep IgG (H&L), ALP conjugated

AS09 628 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)

Product Information

Immunogen

Purified sheep IgG, whole molecule

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified donkey IgG.

Format Liquid

Quantity 1 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (ELISA),1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Sheep IgG heavy and light chains (H&L)

Predicted reactivity Sheep IgG Heavy and Light chains (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative

No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Donkey anti-sheep IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.

Product citations

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