Donkey anti-Sheep IgG (H&L), ALP conjugated
AS09 628 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)
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Product Information
Immunogen
Purified sheep IgG, whole molecule
Host
Donkey
Clonality
Polyclonal
Purity
Purified donkey IgG
Format
Liquid
Quantity
1 mg
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (ELISA),1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Reactivity
Confirmed reactivity
Sheep IgG heavy and light chains (H&L)
Predicted reactivity
Sheep IgG Heavy and Light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
APL conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Background
Background
Donkey anti-sheep IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.
Product citations
Selected references
to be added when available
Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | Fax: +46(0)935 33044 | 
