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Donkey anti-Sheep IgG (H&L), ALP conjugated, min. cross-reactivity to human, mouse,rabbit IgG

AS10 1179  | Clonality: Polyclonal  |  Host:Donkey   |  Reactivity:Sheep IgG (H&L)

Donkey anti-Sheep IgG (H&L), ALP conjugated, min. cross-reactivity to human, mouse,rabbit IgG  in the group Human/Animal Antibodies / Secondary Antibodies/Blocking / Anti-sheep  / HRP, Alkaline phosphatase at Agrisera AB (Antibodies for research) (AS10 1179)

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product information
Background Donkey anti-sheep IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit IgG and are affinity purified using solid phase sheep IgG.
Host Donkey
Clonality Polyclonal
Clone
Purity Affinity purified IgG
Format Liquid
Quantity 1 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products other ALP conjugated secondary antibodies to sheep IgG
Additional information

This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

application information
Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references


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