Donkey anti-Sheep IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse,rabbit IgG

Name: Donkey anti-Sheep IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse,rabbit IgG
SKU: AS10 1179
Price: 239 EUR
Availability: InStock

AS10 1179 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Donkey anti-Sheep IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse,rabbit IgG in the group Secondary Antibodies / Anti-Sheep / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS10 1179)

Product Information

Host Donkey

Clonality Polyclonal

Purity Immunogen affinity purified IgG.

Format Liquid

Quantity 1 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Background Donkey anti-sheep IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit IgG and are affinity purified using solid phase sheep IgG.

Product citations

Related products: Donkey anti-Sheep IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse,rabbit IgG

Donkey anti-Sheep IgG (H&L), Affinity purified, Unconjugated, min, cross-reactivity to human, rabbit IgG

AS10 1293 | Clonality: Polyclonal | Host:Donkey | Reactivity: Sheep IgG (...

185 €

Donkey anti-Sheep IgG (H&L), Biotin conjugated, min, cross-reactivity to human, rabbit IgG

AS10 1147 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG ...

233 €

Donkey anti-Sheep IgG (H&L), DyLight® 488 conjugated

AS09 631 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)

206 €

Donkey anti-Sheep IgG (H&L), FITC conjugated

AS10 1171 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG ...

233 €

Host:	Donkey
Clonality:	Polyclonal
Purity:	Immunogen affinity purified IgG.
Format:	Liquid
Quantity:	1 mg
storage:	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
recommended dilution:	1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)
additional information:	This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis. Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
background:	Donkey anti-sheep IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit IgG and are affinity purified using solid phase sheep IgG.