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Anti-E Tag (monoclonal, clone 11H12B3)
Product no: AS25 5110
AS25 5110 | Clonality: Monoclonal | Host: Mouse | Reactivity: E-tagged proteins
169
€
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Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated synthetic peptide GAPVPYPDPLEPRC Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: >95%, Protein G purified Format: Liquid Quantity: 50 µl Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.Tested applications: ELISA (ELISA), Western Blot (WB), Immunoprecipitation (IP) Recommended dilution: 1:4000-1:8000 (ELISA), 1:1000-1:32 0000 (WB), 1µg-5 µg (IP) Expected | apparent MW: Depends upon MW of fusion partner - Application Examples
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Western Blot: Positive WB detected in: E Tagged fusion protein at 50 ng, 2 5ng, 12.5 ng, 6.25 ng, 3.125 ng
E Tag antibody was used at 1:1000 dilution
Secondary: Goat polyclonal to Mouse IgG at 1:10000 dilution
Calculated and apparent MW: 36 kDa
Immunoprecipitating E Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1 µg) instead of E-tag antibody in 293F transfected whole cell lysate. For Western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at the dilution of 1:2000.
Lane 2: Primary antibody (5µg) + 293F transfected whole cell lysate (500 µg)
Lane 3: 293F transfected whole cell lysate (20 µg)
- Additional Information
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Additional information (application): Antibody is present in 0.01M PBS, pH 7.4, containing 0.03% and 50% glycerol. Proclin 300 is included as preservative - Background
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Background: E-tag monoclonal antibody is designed to specifically recognize and bind to the E-tag peptide. The E-tag is a short peptide sequence used in recombinant protein expression to facilitate the detection, purification, and characterization of tagged proteins. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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