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Anti-E Tag Monoclonal Antibody
Product no: AS25 5110
AS25 5110 | Clonality: Monoclonal | Host: Mouse | Reactivity: E-tagged proteins
169
€
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Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated synthetic peptide GAPVPYPDPLEPRC Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: >95%, Protein G purified Format: Liquid Quantity: 50 µl Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.Tested applications: ELISA (ELISA), Western Blot (WB), Immunoprecipitation (IP) Recommended dilution: 1:4000-1:8000 (ELISA), 1:1000-1:32 0000 (WB), 1µg-5 µg (IP) Expected | apparent MW: Depends upon MW of fusion partner - Application Examples
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Western Blot: Positive WB detected in: E Tagged fusion protein at 50 ng, 2 5ng, 12.5 ng, 6.25 ng, 3.125 ng
E Tag antibody was used at 1:1000 dilution
E tagged fusion protein was subjected to Tris-Glycine 12 % SDS-PAGE gel electrophoresis in reduced conditions at 40 mA followed by a semi-dry transfer at 60 mA for 80 minutes (0.45mm PVDF membrane purchased from Millipore). Blocking was done with 5% skim milk in PBS at RT/1h. Primary antibody was diluted in 0.05% PBST + 2.5% skim milk powder and incubated ON/4°C in a dilution of 1: 5000. Washing was done with 0.1% PBST (4 times, 10 minutes each) followed by a secondary antibody was diluted to 1:10 000 (0.05% with PBST + 2.5% skim milk powder) and incubated at RT/2h followed by four time washes with 0.1% PBST for 10 minutes each and chemiluminescent reaction development and recording of a signal.
Calculated and apparent MW: 36 kDa
Immunoprecipitating E Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1 µg) instead of E-tag antibody in 293F transfected whole cell lysate. For Western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at the dilution of 1:2000.
Lane 2: Primary antibody (5µg) + 293F transfected whole cell lysate (500 µg)
Lane 3: 293F transfected whole cell lysate (20 µg) - Additional Information
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Additional information (application): Antibody is present in 0.01M PBS, pH 7.4, containing 0.03% and 50% glycerol. Proclin 300 is included as preservative - Background
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Background: E-tag monoclonal antibody is designed to specifically recognize and bind to the E-tag peptide. The E-tag is a short peptide sequence used in recombinant protein expression to facilitate the detection, purification, and characterization of tagged proteins. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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