Visar bild 1 av
1
ECFP Tag Monoclonal Antibody
Product no: AS25 5107
AS25 5107 | Clonality: Monoclonal | Host: Mouse | Reactivity: ECFP-tagged proteins
169
€
Customer reviews
Delivery:
3-6 business days
- Product Info
-
Sub class: IgG Immunogen: Recombinant full length ECFP Protein Host: Mouse Clonality: Monoclonal Purity: Affinity purified in PBS pH 7.4 and 50 % glycerol. Contains 0.02 % sodium azide. Format: Liquid Quantity: 50 µg Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.Tested applications: ELISA (ELISA), Western Blot (WB) Recommended dilution: 1:500-1:5000 (WB) Expected | apparent MW: Depends upon MW of fusion partner - Reactivity
-
Confirmed reactivity: ECFP-tagged proteins - Application Examples
-
2 µg ECFP recombinant protein was loaded/well and separated on Tris-Glycine 12 % SDS-PAGE in reduced conditions at 40 mA followed by a semi-dry transfer at 60 mA for 80 minutes (0.45mm PVDF membrane purchased from Millipore). Blocking was done with 5% skim milk in PBS at RT/1h. Primary antibody was diluted in 0.05% PBST + 2.5% skim milk powder and incubated ON/4°C in a dilution of 1: 5000 and 1: 10 000 respectively. Washing was done with 0.1% PBST (4 times, 10 minutes each) followed by a secondary antibody was diluted to 1:50 000 (0.05% with PBST + 2.5% skim milk powder) and incubated at RT/2h followed by four time washes with 0.1% PBST for 10 minutes each and chemiluminescent reaction development and recording of a signal. - Additional Information
-
Additional information (application): Antibody is present in PBS, pH 7.4, containing 0.5% BSA and 50% Glycerol. Contains 0.02% sodium azide as preservative. - Background
-
Background: ECFP tag monoclonal antibody is designed to specifically recognize and bind to the ECFP (Enhanced Cyan Fluorescent Protein) tag. ECFP is a variant of the green fluorescent protein (GFP) that emits cyan fluorescence and is commonly used as a fusion tag in recombinant protein expression to facilitate the detection and visualization of tagged proteins. ECFP is a genetically engineered mutant of GFP which is significantly brighter and folds more efficiently. - Protocols
-
Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
-
This product doesn't have any reviews.
Accessories
AS11 1772 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG (H&L)
182 €
AS20 4485 | Clonality: Monoclonal | Host: Mouse | Reactivity: BFP, GFP, YFP
263 €
AS20 4443 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Green Fluorescence Protein (GFP)
329 €