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Fd3 | Ferredoxin 3

AS20 4432 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana, Zea mays
Fd3 | Ferredoxin 3 in the group Antibodies for Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS20 4432)

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Product Information

Immunogen Purified full length, tag cleaved, recombinant maize Fd3, UniProt: P27788

Host Rabbit
Clonality Polyclonal
Purity Total IgG, purified on Protein A
Format Liquid at 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Quantity 100 µg
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1: 2000 - 1: 10 000 (WB)
Expected | apparent MW 16 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity Brachypodium distachyon, Dichanthelium oligosanthes, Hordeum vulgare, Oryza sativa, Panicum hallii, Saccharum sp. , Setaria italica
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Western blot using anti plant ferredoxin 3 antibodies
Fd3 purified from leaves of Zea mays, 50 ng was denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.


Western blot using anti-Fd3 antibodies

Total leaf extract of Arabidopsis thaliaa (1) and Zea mays (2), 10 µg denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

The antibody is also recognizing root-type ferredoxins expressed in leaves. 


Western blot using anti-Ferredoxin 3 antibodies

Different RNAi (lane 1-7) expressed in T1 Arabidopsis thaliana plants and wild type without any RNAi epxression. were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE. For IP, 150mM NaCL, 1% Triton X-100, 50 mM Tris-HCl (pH 8.0) and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

Additional information

Antibody reacts weakly with other ferredoxins: Arabidopsis thaliana and Zea mays Fd2 and Zea mays Fd6.

Related products

Related products AS20 4434 | Anti-Fd1 | Ferredoxin 1 (chloroplastic), rabbit antibodies
AS20 4433 | Anti-Fd2 | Ferredoxin 2 (chloroplastic), rabbit antibodies

collection of antibodies involved in photosynthesis

Background

Background Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Fd3 is non-photosynthetic Fd expressed more in root than in leaf. Fd3 is localised in chloroplast and plastids.

Product citations

Selected references Hanke and Hase (2008). Variable Photosynthetic Roles of Two Leaf-Type Ferredoxins in Arabidopsis, as Revealed by RNA Interference. Photochem Photobiol. 84(6):1302-9. doi: 10.1111/j.1751-1097.2008.00411.x.
Hanke et al. (2003). A Post Genomic Characterization of Arabidopsis Ferredoxins. Plant Physiol. 134(1):255-64. doi: 10.1104/pp.103.032755.
Matsumura et al. (1997). A Nitrate-Inducible Ferredoxin in Maize Roots. Genomic Organization and Differential Expression of Two Nonphotosynthetic Ferredoxin Isoproteins. Plant Physiol. 114(2):653-60. doi: 10.1104/pp.114.2.653.

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