FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239)

Name: FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239)
Brand: IKP
SKU: AS18 4215
Price: 476 EUR
Availability: InStock

AS18 4215 | Clonality: Monoclonal | Host: Mouse | Reactivity: FITC molecule

FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239) in the group Tag Antibodies / FITC/TRITC at Agrisera AB (Antibodies for research) (AS18 4215)

Product Information

Immunogen Highly purified fluorescein isothiocyanate isomer 1,

Host Mouse

Clonality Monoclonal

Purity Purified IgG.1 in PBS pH 7.2, HRP conjugated.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 0,5 ml of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.

Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Dot blot (Dot), Western blot (WB)

Recommended dilution The optimum working dilution should be determined by titration by end user.
1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).

Reactivity

Confirmed reactivity FITC molecule

Application examples

Additional information

Additional information This product is intended FOR RESEARCH USE ONLY.

Peroxidase/IgG protein molar ratio (E/P) approximately 1.7.

This product has not been tested for cross-reactivity with other fluorescent compounds

Background

FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.

Product citations

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immunogen:	Highly purified fluorescein isothiocyanate isomer 1,
Reconstitution:	For reconstitution add 0,5 ml of sterile water
Host:	Mouse
Clonality:	Monoclonal
Purity:	Purified IgG.1 in PBS pH 7.2, HRP conjugated.
Format:	Lyophilized
Quantity:	200 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
tested applications:	ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Dot blot (Dot), Western blot (WB)
recommended dilution:	The optimum working dilution should be determined by titration by end user. 1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).
Confirmed reactivity:	FITC molecule
additional information:	This product is intended FOR RESEARCH USE ONLY. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7.
additional information (application):	This product has not been tested for cross-reactivity with other fluorescent compounds
background:	FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.