FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239)

Product no: AS18 4215

AS18 4215 | Clonality: Monoclonal | Host: Mouse | Reactivity: FITC molecule

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  • Product Info
  • Immunogen: Highly purified fluorescein isothiocyanate isomer 1
    Host: Mouse
    Clonality: Monoclonal
    Purity: Purified IgG.1 in PBS pH 7.2, HRP conjugated.
    Format: Lyophilized
    Quantity: 200 µg
    Reconstitution: For reconstitution add 0.5 ml of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
    Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) paraffin, Dot blot (Dot), Western blot (WB)
    Recommended dilution: The optimum working dilution should be determined by titration by end user.
    1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).
  • Reactivity
  • Confirmed reactivity: FITC molecule
  • Additional Information
  • Additional information: This product is intended FOR RESEARCH USE ONLY.

    Peroxidase/IgG protein molar ratio (E/P) approximately 1.7.
    Additional information (application): This product has not been tested for cross-reactivity with other fluorescent compounds
  • Background
  • Background:

    FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.

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