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FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239)

AS18 4215 | Clonality: Monoclonal | Host: Mouse | Reactivity: FITC molecule

FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239) in the group Tag Antibodies / FITC at Agrisera AB (Antibodies for research) (AS18 4215)

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341
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Product Information

Immunogen

Highly purified fluorescein isothiocyanate isomer 1.

Host Mouse
Clonality Monoclonal
Purity HRP conjugated purified mouse IgG1 in phosphate buffered saline (pH 7.2).
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 0.5 ml of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Stort time storage at +4°C.
Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Dot blot (Dot), Western blot (WB)
Recommended dilution The optimum working dilution should be determined by titration by end user.
1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).

Reactivity

Confirmed reactivity FITC molecule

Application examples

Additional information

Additional information This product is intended FOR RESEARCH USE ONLY.

Peroxidase/IgG protein molar ratio (E/P) approximately 1.7.

This product has not been tested for cross-reactivity with other fluorescent compounds.

Related products

Related products other tag antibodies

Background

Background

FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.

Product citations

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