FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239)
AS18 4215 | Clonality: Monoclonal | Host: Mouse | Reactivity: FITC molecule
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1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).
Peroxidase/IgG protein molar ratio (E/P) approximately 1.7.
FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.