FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239)
AS18 4215 | Clonality: Monoclonal | Host: Mouse | Reactivity: FITC molecule
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Product Information
Immunogen
Highly purified fluorescein isothiocyanate isomer 1.
Host
Mouse
Clonality
Monoclonal
Purity
HRP conjugated purified mouse IgG1 in phosphate buffered saline (pH 7.2).
Format
Lyophilized
Quantity
200 µg
Reconstitution
For reconstitution add 0.5 ml of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Stort time storage at +4°C.
Tested applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Dot blot (Dot), Western blot (WB)
Recommended dilution
The optimum working dilution should be determined by titration by end user.
1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).
1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).
Reactivity
Confirmed reactivity
FITC molecule
Application examples
Additional information
Additional information
This product is intended FOR RESEARCH USE ONLY.
Peroxidase/IgG protein molar ratio (E/P) approximately 1.7.
Peroxidase/IgG protein molar ratio (E/P) approximately 1.7.
This product has not been tested for cross-reactivity with other fluorescent compounds.
Background
Background
FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
Product citations
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