FITC | fluorescein isothiocyanate (monoclonal, Clone NI 239)

AS18 4213 | Clonality: Monoclonal | Host: Mouse | Reactivity: FITC molecule

Product Information

Immunogen Highly purified fluorescein isothiocyanate isomer 1,

Host Mouse

Clonality Monoclonal

Purity Purified IgG.1 in PBS pH 7.2.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 0,5 ml of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.

Tested applications ELISA (ELISA), Indirect immunofluorescence (IIF)

Recommended dilution The optimum working dilution should be determined by titration by end user.
1:500 (ELISA), 1:50 to 1:200 (IL)

Reactivity

Confirmed reactivity FITC molecule

Application examples

Additional information

Additional information This product is intended FOR RESEARCH USE ONLY

This product has not been tested for cross-reactivity with other fluorescent compounds

Background

FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.

Product citations

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