Rabbit anti-Mouse IgG (H&L), HRP conjugated

AS09 627 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L)

Rabbit anti-Mouse IgG (H&L), HRP conjugated in the group Secondary Antibodies / Anti-Mouse / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS09 627)
Rabbit anti-Mouse IgG (H&L), HRP conjugated


How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information


Purified Mouse IgG, whole molecule

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified rabbit IgG.
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)


Confirmed reactivity Mouse IgG heavy and light chains (H&L)
Predicted reactivity Mouse IgG Heavy and Light chains (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-plant actin, mouse recombinant antibodies

10 µg/well of total protein extracted freshly from Arabidopsis thaliana leaf tissue. All lanes shown are from different Arabidopsis thaliana leaf samples extracted simultaneously. Fresh leaf tissue was ground up directly in 1x Bolt LDS loading buffer (Thermo) and 200mM DTT and denatured at 80°C for 10 min. Samples were separated on 12% SDS-PAGE gel and transferred to nitrocellulose by wet transfer for 1hr at 100V. Blot was blocked with 5 % milk in TBST for 1h/RT with agitation. Blot was incubated with Agrisera Mouse anti-actin monoclonal (AS21 4615) at a dilution of 1:1000, in 5% milk with agitation at 1h RT. The blot was rinsed 3 times for 5 minutes in TBS-T with agitation. Then the membrane was incubated with secondary anti-mouse HRP (Agrisera AS09 627) at 1:10 000 dilution in milk for 1hr at room temp. The blots were washed as above and reaction was visualized using ECL reagent and following manufacture's recommendations. The actin band was visualized after 15 seconds of film exposure.

Additional information

Additional information

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis

Related products



Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.

Product citations

Selected references Vitale et al. (2021) Light Spectral Composition Influences Structural and Eco-Physiological Traits of Solanum lycopersicum L. cv. 'Microtom' in Response to High-LET Ionizing Radiation. Plants (Basel). 2021 Aug 23;10(8):1752. doi: 10.3390/plants10081752. PMID: 34451797; PMCID: PMC8399554.
Bui et al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. BioRxiv.
Koch et al. (2019). Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine. Proc Natl Acad Sci U S A. 2019 May 21;116(21):10333-10338. doi: 10.1073/pnas.1820130116.

Related products: Rabbit anti-Mouse IgG (H&L), HRP conjugated

AS10 820 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L)
206 €
AS09 629 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L...
233 €
AS10 1149   | Clonality: Polyclonal  |  Host: Rabbit   |  Reactivity: Mou...
246 €
AS10 957 | Clonality: Polyclonal  | Host: Rabbit | Reactivity: Mouse IgG (H&L)
175 €