showForm Rabbit anti-mouse IgG (H&L) HRP conjugated secondary antibody

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Rabbit anti-Mouse IgG (H&L), HRP conjugated

AS09 627 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L)

Rabbit anti-Mouse IgG (H&L), HRP conjugated in the group Secondary Antibodies / Anti-Mouse / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS09 627)

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Product Information

Immunogen

Purified Mouse IgG, whole molecule

Host Rabbit
Clonality Polyclonal
Purity Purified rabbit IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)

Reactivity

Confirmed reactivity Mouse IgG heavy and light chains (H&L)
Predicted reactivity Mouse IgG Heavy and Light chains (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis

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Background

Background

Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.

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