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Rabbit anti-Mouse IgG (H&L), TRITC conjugated

AS10 957 | Clonality: Polyclonal  | Host: Rabbit | Reactivity: Mouse IgG (H&L)

Rabbit anti-Mouse IgG (H&L), TRITC conjugated in the group Secondary Antibodies / Anti-Mouse / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS10 957)

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Product Information

Host Rabbit
Clonality Polyclonal
Purity Affinity purified rabbit IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution The optimal working dilution should be determined by the investigator. (ICC), immunohistochemistry ( IHC) (Flow cyt)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

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Background

Background Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to Rhodamine (TRITC-rhodamine - Tetramethylrhodamine-5-isothiocyanate)  which is reacting with mouse IgG (H&L) in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase mouse IgG.

Product citations

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