Rabbit anti-Mouse IgG (H&L), ALP conjugated
AS09 629 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)
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Product Information
Immunogen
Purified Mouse IgG, whole molecule.
Host
Rabbit
Clonality
Polyclonal
Purity
Purified rabbit IgG
Format
Liquid, clear, colorless.
Quantity
1 mg
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Shelf life is one year from date of receipt.
Shelf life is one year from date of receipt.
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB)
Recommended dilution
1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Reactivity
Confirmed reactivity
Mouse IgG heavy and light chains (H&L).
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.
Based on IEP, this antibody binds to:
heavy chains on mouse IgG
light chains on all mouse immunoglobulins
No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.
Background
Background
Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all mouse immunoglobulins in immunological assays.
Product citations
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