Rabbit anti-Mouse IgG (H&L), ALP conjugated

AS09 629 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Product Information

Immunogen Purified Mouse IgG, whole molecule,

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified rabbit IgG.

Format Liquid, clear, colorless.

Quantity 1 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB)

Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Mouse IgG heavy and light chains (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.

Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)

Based on IEP, this antibody binds to:

heavy chains on mouse IgG
light chains on all mouse immunoglobulins

No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.

Background

Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all mouse immunoglobulins in immunological assays.

Product citations

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Confirmed reactivity:	Mouse IgG heavy and light chains (H&L)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Clonality:	Polyclonal
Format:	Liquid, clear, colorless.
Host:	Rabbit
immunogen:	Purified Mouse IgG, whole molecule,
Purity:	Immunogen affinity purified rabbit IgG.
Quantity:	1 mg
recommended dilution:	1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
storage:	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB)
background:	Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all mouse immunoglobulins in immunological assays.
additional information:	ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative. Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)
additional information (application):	Based on IEP, this antibody binds to: heavy chains on mouse IgG light chains on all mouse immunoglobulins No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.