Rabbit anti-Mouse IgG (H&L), ALP conjugated

AS09 629 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)


product information
Background		Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all mouse immunoglobulins in immunological assays.
Immunogen		Purified Mouse IgG, whole molecule.
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Purified rabbit IgG
Format		Liquid, clear, colorless.
Quantity		1 mg
Reconstitution
Storage		Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Shelf life is one year from date of receipt.
Tested applications		ELISA (ELISA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB)
Related products
Additional information		ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.

application information
Recommended dilution		1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Expected \| apparent MW
Confirmed reactivity		Mouse IgG heavy and light chains (H&L).
Predicted reactivity
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		Based on IEP, this antibody binds to: heavy chains on mouse IgG light chains on all mouse immunoglobulins No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.
Selected references

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com