Rabbit anti-Mouse IgG (H&L), ALP conjugated

AS09 629 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)

Product Information

Immunogen

Purified Mouse IgG, whole molecule.

Host Rabbit

Clonality Polyclonal

Purity Purified rabbit IgG

Format Liquid, clear, colorless.

Quantity 1 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Shelf life is one year from date of receipt.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB)

Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Mouse IgG heavy and light chains (H&L).

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.

Based on IEP, this antibody binds to:

heavy chains on mouse IgG
light chains on all mouse immunoglobulins

No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.

Background

Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all mouse immunoglobulins in immunological assays.