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Anti-FT/TSF | Flowering locus T and twin sister of FT
AS06 198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Oryza sativa
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived fromA.thaliana FT protein sequence(Q9SXZ2, At1g65480); please note that this antibody will cross-react with the highly homologous TSF (twin sister of FT) protein
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 20 | 20 kDa (Arabidopsis thaliana)
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Oryza sativa Predicted reactivity: Betula luminifera, Brassica napus, Brassica oleracea, Brassica rapa, Citrus sinensis, Eucalyptus sp., Hordeum vulgare, Jatropha curcas, Nicotiana tabacum, Persea americana var. americana, Populus tomentosa, Prunus armeniaca, Prunus avium, Prunus dulcis, Prunus mume, Prunus persica,Solanum tuberosum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: Medicago truncatula, Pisum sativum - Application Examples
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Application example
35 µg of total leaf protein extracted with PEB (AS08 300) from wt Arabidopsis thaliana (1) and Arabidopsis thaliana transformed with 35S::FT (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to PVDF. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FT/TFT (AS06 198, 1:1000, 1h) and secondary anti-rabbit (1:20 000, 1h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (300s, high sensitivity). Please note that this antibody will not detect FT at 35 µg protein loading in the wt leaf material tested.
Arabidopsis thaliana plants were 4 weeks old, grown @ 8 h light with 130-150 µE light @22°C and 16 h dark @18°C. - Additional Information
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Additional information (application): Note that detection for this product is limited by target threshold level - Background
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Background: In angioperms the 20 kDa protein FT (coded by the gene flowering locus T) promotes flowering under long-day conditions. It has been shown to contribute to flowering initiation upon its expression in leafs followed by transport to apical meristems.
- Product Citations
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Selected references: Nakamura et al. (2019). High-Resolution Crystal Structure of Arabidopsis FLOWERING LOCUS T Illuminates Its Phospholipid-Binding Site in Flowering. iScience. 2019 Nov 22;21:577-586. doi: 10.1016/j.isci.2019.10.045.
Liang and Ow et al. (2019). Nucleocytoplasmic OXIDATIVE STRESS 2 can relocate FLOWERING LOCUS T. Biochemical and Biophysical Research Communications Volume 517, Issue 4, 1 October 2019, Pages 735-740 - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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