FT/TSF | Flowering locus T and twin sister of FT

KLH-conjugated synthetic peptide derived fromA.thaliana FT protein sequence(Q9SXZ2, At1g65480); please note that this antibody will cross-react with the highly homologous TSF (twin sister of FT) protein

20 | 20 kDa (Arabidopsis thaliana)

Application example

35 µg of total leaf protein extracted with PEB (AS08 300) from wt Arabidopsis thaliana (1) and Arabidopsis thaliana transformed with 35S::FT (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to PVDF. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FT/TFT (AS06 198, 1:1000, 1h) and secondary anti-rabbit (1:20 000, 1h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (300s, high sensitivity). Please note that this antibody will not detect FT at 35 µg protein loading in the wt leaf material tested.

AS16 3160 | Anti-CO | Constans, rabbit antibodies
AS12 1864 | Anti-GI | Gigantea, rabbit antibodies
AS12 1864A | Anti-GI | Gigantea, rabbit antibodies
AS12 2646 | Anti-TOC1 | Timing of CAB expression 1, rabbit antibodies
AS14 2823 | Anti-TOC1 | Timing of CAB expression 1 (other species), rabbit antibodies
AS12 1856 | Anti-SOC1 | Suppressor of constans overexpression 1, rabbit antibodies

In angioperms the 20 kDa protein FT (coded by the gene flowering locus T) promotes flowering under long-day conditions. It has been shown to contribute to flowering initiation upon its expression in leafs followed by transport to apical meristems.