FT/TSF | Flowering locus T and twin sister of FT

AS06 198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


product information
Background		In angioperms the 20 kDa protein FT (coded by the gene flowering locus T) promotes flowering under long-day conditions. It has been shown to contribute to flowering initiation upon its expression in leafs followed by transport to apical meristems.
Immunogen		KLH-conjugated synthetic peptide derived fromA.thaliana FT protein sequence(Q9SXZ2, At1g65480); please note that this antibody will cross-react with the highly homologous TSF (twin sister of FT) protein
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinity purified serum
Format		Lyophilized
Quantity		50 µg
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		AS16 3160 \| anti-CO \| Constans, rabbit polyclonal antibody AS12 1864 \| anti-GI \| Gigantea, rabbit polyclonal antibody AS12 1864A \| anti-GI \| Gigantea, rabbit polyclonal antibody AS12 2646 \| anti-TOC1 \| Timing of CAB expression 1, rabbit polyclonal antibody AS14 2823 \| anti-TOC1 \| Timing of CAB expression 1 (other species), rabbit polyclonal antibody AS12 1856 \| anti-SOC1 \| Suppressor of constans overexpression 1, rabbit polyclonal antibody
Additional information

application information
Recommended dilution		1 : 1000 (WB)
Expected \| apparent MW		20 \| 20 kDa for Arabidopsis thaliana
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity		Betula luminifera, Brassica napa, Brassica oleracea, Hordeum vulgare, Oryza sativa, Populus tomentosa, Solanum tuberosum, Zea mays
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		Note that detection for this product is limited by target threshold level.
Selected references

application example

35 µg of total leaf protein extracted with PEB (AS08 300) from wt Arabidopsis thaliana (1) and Arabidopsis thaliana transformed with 35S::FT (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to PVDF. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FT/TFT (AS06 198, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (300s, high sensitivity). Please note that this antibody will not detect FT at 35 µg protein loading in the wt leaf material tested.

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