FT/TSF | Flowering locus T and twin sister of FT

AS06 198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide derived fromA.thaliana FT protein sequence(Q9SXZ2, At1g65480); please note that this antibody will cross-react with the highly homologous TSF (twin sister of FT) protein

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS pH 7.4

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

20 | 20 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Betula luminifera, Brassica napa, Brassica oleracea, Eucalyptus sp., Hordeum vulgare, Jatropha curcas, Nicotiana tabacum, Oryza sativa, Persea americana var. americana, Populus tomentosa, Solanum tuberosum, Zea mays
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

35 µg of total leaf protein extracted with PEB (AS08 300) from wt Arabidopsis thaliana (1) and Arabidopsis thaliana transformed with 35S::FT (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to PVDF. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FT/TFT (AS06 198, 1:1000, 1h) and secondary anti-rabbit (1:20 000, 1h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (300s, high sensitivity). Please note that this antibody will not detect FT at 35 µg protein loading in the wt leaf material tested.

Arabidopsis thaliana plants were 4 weeks old, grown @ 8 h light with 130-150 µE light @22°C and 16 h dark @18°C.

Additional information

Note that detection for this product is limited by target threshold level.

Related products

Background

In angioperms the 20 kDa protein FT (coded by the gene flowering locus T) promotes flowering under long-day conditions. It has been shown to contribute to flowering initiation upon its expression in leafs followed by transport to apical meristems.

Product citations

Selected references Nakamura et al. (2019). High-Resolution Crystal Structure of Arabidopsis FLOWERING LOCUS T Illuminates Its Phospholipid-Binding Site in Flowering. iScience. 2019 Nov 22;21:577-586. doi: 10.1016/j.isci.2019.10.045.
Liang and Ow et al. (2019). Nucleocytoplasmic OXIDATIVE STRESS 2 can relocate FLOWERING LOCUS T. Biochemical and Biophysical Research Communications Volume 517, Issue 4, 1 October 2019, Pages 735-740