FT/TSF | Flowering locus T and twin sister of FT
AS06 198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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KLH-conjugated synthetic peptide derived fromA.thaliana FT protein sequence(Q9SXZ2, At1g65480); please note that this antibody will cross-react with the highly homologous TSF (twin sister of FT) protein
20 | 20 kDa (Arabidopsis thaliana)
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35 µg of total leaf protein extracted with PEB (AS08 300) from wt Arabidopsis thaliana (1) and Arabidopsis thaliana transformed with 35S::FT (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to PVDF. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FT/TFT (AS06 198, 1:1000, 1h) and secondary anti-rabbit (1:20 000, 1h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (300s, high sensitivity). Please note that this antibody will not detect FT at 35 µg protein loading in the wt leaf material tested.
Arabidopsis thaliana plants were 4 weeks old, grown @ 8 h light with 130-150 µE light @22°C and 16 h dark @18°C.
In angioperms the 20 kDa protein FT (coded by the gene flowering locus T) promotes flowering under long-day conditions. It has been shown to contribute to flowering initiation upon its expression in leafs followed by transport to apical meristems.
Liang and Ow et al. (2019). Nucleocytoplasmic OXIDATIVE STRESS 2 can relocate FLOWERING LOCUS T. Biochemical and Biophysical Research Communications Volume 517, Issue 4, 1 October 2019, Pages 735-740
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