GAPDH | Glyceraldehyde-3-phosphate dehydrogenase (chloroplastic)

Product no: AS22 4848

AS22 4848 | Clonality: Polyclonal|  Host: Rabbit | Reactivity:  Spinacia oleracea

Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana GAPDH, UniProt: P25857, TAIR: At1g42970
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 42.4 | 36.3 kDa (due to N-terminal processing)
  • Reactivity
  • Confirmed reactivity: Spinacia oleracea
    Predicted reactivity: Arabidopsis thaliana, Brassica napus, Nicotiana tabacum, Solanum lycopresicum, Solanum tuberosum, Pisum sativum, Hordeum vulgare, Oryza sativa, Zea mays

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-GAPDH antibodies (chloroplastic)

    Variable amounts of purified spinach AB-GAPDH enzyme (0.32, 0.8, 2, and 5 µg/well) and protein extract (8, 20, 50 and 125 µg/well) from Spinacia oleracea leaves were separated on 12% SDS-PAGE and blotted to a nitrocellulose membrane (0.2 µm) using the TransBlot (BioRad). Membrane was stained with Red-Ponceau and then blocked with TBST containing 5% skim milk for 1 h at RT. After blocking, the membrane was incubated at 4°C/ON in the presence of Agrisera primary antibody (1:1 000 dilution) in TBST containing 1% milk under continuous agitation. After incubation, the membrane was washed 5 times for 10 min in TBST with 1% milk at RT and then incubated with Agrisera secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) at 1:25 000 dilution for 90 min at RT. The membrane was then washed 5 times for 10 min in TBST with 1% milk and TBST for an additional 20 min prior to incubation (2 min) with Agrisera ECLBright (AS16 ECL-N). The signal was acquired using the LAS500 instrument (Cytiva) after exposure times of 30 and 80 sec. ​ ​
    The GAPDH enzyme was purified according to Fermani et al. (2007)

    Courtesy of Dr. Aitziber Calleja, University of Navarra, Spain

  • Additional Information
  • Additional information: Purified chloroplast sample is recommended to be used with this antibody.
  • Background
  • Background: GAPDH (Glyceraldehyde-3-phosphate dehydrogenase (chloroplastic)  is an enzyme involved in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). Protein belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.


AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart

AS05 084 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane

Benefits of using this antibody

319 €
Add to cart
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS03 037 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: global antibody  and compartment marker for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms 

Benefits of using this antibody

319 €
Add to cart
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
img missing
AS16 ECL-S-N-100 | Low pico to mid femtogram and extreme low femtogram detection
222 €