GDH2 | Glutamate dehydrogenase 2

Product no: AS16 4034

AS16 4034   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Lupinus luteus

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  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide derived from GDH2 from Lupinus luteus
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl, of sterile water
    Storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Recommended dilution:

    1 : 1000 (WB)

    Expected | apparent MW: 44.7 kDa
  • Reactivity
  • Confirmed reactivity: Lupinus luteus 
    Predicted reactivity: Hordeum vulgare, Zea mays

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  • Application Examples
  • Application example

    Western blot using anti-GDH2 antibodies

    Purified, recombinant GDH1 and GDH2 subunits and whole GDH1 from Lupinus luteus were separated on 12 % SDS-PAGE  and blotted 1h to nitrocellulose membrane. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. Antibody binding was visualized through STRAP/HRP with 2-amino benzidine.

    Courtesy of Dr. Teresa Lehmann, Adam Mickiewicz University, Poland

  • Background
  • Background:
    GDH2 (glutamate dehydrogenase 2) is a beta-subunit of the glutamate dehydrogenase, an enzyme found mainly in the mitochondria of stem and leaf companion cells.
  • Product Citations
  • Selected references: Lehmann et al. (2011). Organ-specific expression of glutamate dehydrogenase (GDH) subunits in yellow lupine. J Plant Physiol. 2011 Jul 1;168(10):1060-6. doi: 10.1016/j.jplph.2010.12.016.
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items