GDH2 | Glutamate dehydrogenase 2
AS16 4034 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Lupinus luteus
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For reconstitution add 50 µl, of sterile water.
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
1 : 1000 (WB)
Purified, recombinant GDH1 and GDH2 subunits and whole GDH1 from Lupinus luteus were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. Antibody binding was visualized through STRAP/HRP with 2-amino benzidine.
Courtesy of Dr. Teresa Lehmann, Adam Mickiewicz University, Poland