GDH2 | Glutamate dehydrogenase 2

AS16 4034 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Lupinus luteus

Product Information

Immunogen KLH-conjugated synthetic peptide derived from GDH2 from Lupinus luteus

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW 44,7 kDa

Reactivity

Confirmed reactivity Lupinus luteus

Predicted reactivity Hordeum vulgare, Zea mays
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Application examples

Application examples Application example

Western blot using anti-GDH2 antibodies

Purified, recombinant GDH1 and GDH2 subunits and whole GDH1 from Lupinus luteus were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. Antibody binding was visualized through STRAP/HRP with 2-amino benzidine.

Courtesy of Dr. Teresa Lehmann, Adam Mickiewicz University, Poland

Additional information

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Background

GDH2 (glutamate dehydrogenase 2) is a beta-subunit of the glutamate dehydrogenase, an enzyme found mainly in the mitochondria of stem and leaf companion cells.

Product citations

Selected references Lehmann et al. (2011). Organ-specific expression of glutamate dehydrogenase (GDH) subunits in yellow lupine. J Plant Physiol. 2011 Jul 1;168(10):1060-6. doi: 10.1016/j.jplph.2010.12.016.