Goat anti-Chicken IgY (H&L), DyLightŪ 405 conjugated
AS16 3264 | Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (H&L)
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Product Information
Immunogen
Purified Chicken IgY, whole molecule.
Host
Goat
Clonality
Polyclonal
Purity
Affinity purified
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage
Store freeze-dried powder at 2-8 °C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Flow cytometry (Flow cyt), Immunolocalization (IL)
Recommended dilution
1 : 20-1 : 2000 (Flow cyt), (IL)
Reactivity
Confirmed reactivity
Chicken IgY
Application examples
Additional information
Additional information
Antibody purity is > 95% based on SDS-PAGE.
DyLight® 405 (Ex = 400 nm; Em = 420 nm)
Affinity purified using solid phase Chicken IgG/Y.
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.
Based on IEP, this antibody reacts with: • heavy (γ) chains on chicken IgG/Y • light chains on all chicken immunoglobulins .
Based on IEP, no reactivity is observed to: • non-immunoglobulin chicken serum immunoglobulins .
Background
Background
Goat anti-Chicken IgY (H&L) is a secondary antibody, conjugated to DyLight® 405, which binds to chicken IgY (H&L) in immunological assays.
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product citations
Selected references
Huiming et al. (2020). Integration of Nodal and BMP Signaling by Mutual Signaling Effector Antagonism. Cell Rep. 2020 Apr 7;31(1):107487.doi: 10.1016/j.celrep.2020.03.051.
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