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product information
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| Background |
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Goat anti-Chicken IgY (H&L) is a secondary antibody, conjugated to DyLight® 405, which binds to chicken IgY (H&L) in immunological assays.
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries. |
| Immunogen |
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Purified Chicken IgY, whole molecule. |
| Host |
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Goat |
| Clonality |
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Polyclonal |
| Clone |
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| Purity |
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Affinity purified |
| Format |
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Lyophilized |
| Quantity |
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1 mg |
| Reconstitution |
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For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
| Storage |
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Store freeze-dried powder at 2-8 °C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Tested applications |
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Flow cytometry (Flow cyt), Immunolocalization (IL) |
| Related products |
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other anti-chicken DyLight® conjugated secondary antibodies |
| Additional information |
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Antibody purity is > 95% based on SDS-PAGE.
DyLight® 405 (Ex = 400 nm; Em = 420 nm)
Affinity purified using solid phase Chicken IgG/Y.
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.
Based on IEP, this antibody reacts with: • heavy (γ) chains on chicken IgG/Y • light chains on all chicken immunoglobulins .
Based on IEP, no reactivity is observed to: • non-immunoglobulin chicken serum immunoglobulins . |
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