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Goat anti-Dog IgG Fc specific, Unconjugated

AS16 3120 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG

Goat anti-Dog IgG Fc specific, Unconjugated in the group Secondary Antibodies / Anti-Dog / Unconjugated at Agrisera AB (Antibodies for research) (AS16 3120)

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Product Information

Immunogen

purified dog IgG from pooled dog serum

Host Goat
Clonality Polyclonal
Purity Protein G purified IgG
Format Lyophilized
Quantity 1 ml
Reconstitution For reconstitution add 1 ml of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in 4°C up to 7 days.
Tested applications immuniprecipitation (IP)
Recommended dilution

Immunoprecipitation. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Precipitating titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration.

Reactivity

Confirmed reactivity Dog IgG, Fc part
Not reactive in

The antiserum does not cross-react with any other component of the dog immunoglobulin system.

Application examples

Additional information

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of antibody has not been characterized in details.

No preservative added, as it may interfere with the antibody activity.

Related products

Background

Background

Goat anti-Dog IgG Fc, Unconjugated is a secondary antibody which binds to dog IgG FC part in immunological assays.

Product citations

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