Goat anti-Dog IgG Fc specific, Unconjugated

AS16 3120 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG

Product Information

Immunogen

purified dog IgG from pooled dog serum

Host Goat

Clonality Polyclonal

Purity Protein G purified goat IgG.

Format Lyophilized

Quantity 1 ml

Reconstitution For reconstitution add 1 ml of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.

Tested applications immuniprecipitation (IP)

Recommended dilution Immunoprecipitation, In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells, Precipitating titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration

Reactivity

Confirmed reactivity Dog IgG, Fc part

Not reactive in The antiserum does not cross-react with any other component of the dog immunoglobulin system

Application examples

Additional information

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of antibody has not been characterized in details.

No preservative added, as it may interfere with the antibody activity.

Background

Goat anti-Dog IgG Fc, Unconjugated is a secondary antibody which binds to dog IgG FC part in immunological assays.

Product citations

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additional information (application):	Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of antibody has not been characterized in details. No preservative added, as it may interfere with the antibody activity.
Confirmed reactivity:	Dog IgG, Fc part
not reactive in:	The antiserum does not cross-react with any other component of the dog immunoglobulin system
background:	Goat anti-Dog IgG Fc, Unconjugated is a secondary antibody which binds to dog IgG FC part in immunological assays.
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Goat
immunogen:	purified dog IgG from pooled dog serum
Purity:	Protein G purified goat IgG.
Quantity:	1 ml
recommended dilution:	Immunoprecipitation, In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells, Precipitating titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration
Reconstitution:	For reconstitution add 1 ml of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.
tested applications:	immuniprecipitation (IP)