Goat anti-Dog IgG Fc specific, Unconjugated
AS16 3120 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG
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Product Information
Immunogen
purified dog IgG from pooled dog serum
Host
Goat
Clonality
Polyclonal
Purity
Protein G purified IgG
Format
Lyophilized
Quantity
1 ml
Reconstitution
For reconstitution add 1 ml of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes, Reconstituted material can be stoored in 4°C up to 7 days
Tested applications
immuniprecipitation (IP)
Recommended dilution
Immunoprecipitation, In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells, Precipitating titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration
Reactivity
Confirmed reactivity
Dog IgG, Fc part
Not reactive in
The antiserum does not cross-react with any other component of the dog immunoglobulin system
Application examples
Additional information
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of antibody has not been characterized in details.
No preservative added, as it may interfere with the antibody activity.
Background
Background
Goat anti-Dog IgG Fc, Unconjugated is a secondary antibody which binds to dog IgG FC part in immunological assays.
Product citations
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