Goat anti-Dog IgG Fc specific, Unconjugated

AS16 3120 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG


product information
Background		Goat anti-Dog IgG Fc, Unconjugated is a secondary antibody which binds to dog IgG FC part in immunological assays.
Immunogen		purified dog IgG from pooled dog serum
Host		Goat
Clonality		Polyclonal
Clone
Purity		Protein G purified IgG
Format		Lyophilized
Quantity		1 ml
Reconstitution		For reconstitution add 1 ml of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in 4°C up to 7 days.
Tested applications		immuniprecipitation (IP)
Related products		other unconjugated secondary antibodies to dog IgG Secondary antibodies
Additional information

application information
Recommended dilution		Immunoprecipitation. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Precipitating titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration.
Expected \| apparent MW
Confirmed reactivity		Dog IgG, Fc part
Predicted reactivity
Not reactive in		The antiserum does not cross-react with any other component of the dog immunoglobulin system.
Additional information		Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of antibody has not been characterized in details. No preservative added, as it may interfere with the antibody activity.
Selected references		to be added when available, antibody released in January 2016.

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