Goat anti-Dog IgG Fc specific, Biotin conjugated

Name: Goat anti-Dog IgG Fc specific, Biotin conjugated
Brand: IKP
SKU: AS16 3121
Price: 402 EUR
Availability: InStock

AS16 3121 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG Fc specific

Goat anti-Dog IgG Fc specific, Biotin conjugated in the group Secondary Antibodies / Anti-Dog / Biotinylated at Agrisera AB (Antibodies for research) (AS16 3121)

Product Information

Immunogen Purified dog IgG isolated from dog serum.

Host Goat

Clonality Polyclonal

Purity Protein G purified goat IgG.

Format Lyophilized from PBS pH 7.2

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stored in 4°C up to 7 days. Working dilutions should be stored at 4°C and not frozen. If any precipitate occurs, it should be removed by centrifugation.

Tested applications Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)

Recommended dilution 1 : 100-250 (IHC and ICC parrafin sections), 1 : 500-5000 (ELISA), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal

Reactivity

Confirmed reactivity Dog IgG, Fc part

Not reactive in It does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Application examples

Additional information

Additional information The biotin/protein molar ratio is approximately 6.7.

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail.

No preservative added, as it may interfere with the antibody activity.

Background

Goat anti-Dog IgG Fc, Biotin conjugated is a secondary antibody which binds to dog IgG Fc part, in immunological assays.

Product citations

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additional information:	The biotin/protein molar ratio is approximately 6.7.
additional information (application):	Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail. No preservative added, as it may interfere with the antibody activity.
Confirmed reactivity:	Dog IgG, Fc part
not reactive in:	It does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Clonality:	Polyclonal
Format:	Lyophilized from PBS pH 7.2
Host:	Goat
immunogen:	Purified dog IgG isolated from dog serum.
Purity:	Protein G purified goat IgG.
Quantity:	10 mg
recommended dilution:	1 : 100-250 (IHC and ICC parrafin sections), 1 : 500-5000 (ELISA), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
Reconstitution:	For reconstitution add 1 ml of sterile destilled water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stored in 4°C up to 7 days. Working dilutions should be stored at 4°C and not frozen. If any precipitate occurs, it should be removed by centrifugation.
tested applications:	Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
background:	Goat anti-Dog IgG Fc, Biotin conjugated is a secondary antibody which binds to dog IgG Fc part, in immunological assays.