Goat anti-Dog IgG Fc specific, Biotin conjugated

Product no: AS16 3121

AS16 3121 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG Fc specific

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Delivery:  3-6 business days
  • Product Info
  • Immunogen: Purified dog IgG isolated from dog serum.
    Host: Goat
    Clonality: Polyclonal
    Purity: Protein G purified goat IgG.
    Format: Lyophilized from PBS pH 7.2
    Quantity: 10 mg
    Reconstitution: For reconstitution add 1 ml of sterile destilled water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stored in 4°C up to 7 days. Working dilutions should be stored at 4°C and not frozen. If any precipitate occurs, it should be removed by centrifugation.
    Tested applications: Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
    Recommended dilution: 1 : 100-250 (IHC and ICC parrafin sections), 1 : 500-5000 (ELISA), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
  • Reactivity
  • Confirmed reactivity: Dog IgG, Fc part
    Not reactive in: It does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  • Additional Information
  • Additional information: The biotin/protein molar ratio is approximately 6.7.
    Additional information (application):

    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail.

    No preservative added, as it may interfere with the antibody activity.

  • Background
  • Background:

    Goat anti-Dog IgG Fc, Biotin conjugated is a secondary antibody which binds to dog IgG Fc part, in immunological assays.

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